Analysis of T-Lymphocyte Subsets After Phytohemagglutinin Stimulation in Normal and Type 1 Diabetic Mothers and Their Infants

Our aim was to investigate the immunological status of diabetic pregnancy, which is an overlap of diabetic immunity abnormalities and the immunological modifications normally occurring during pregnancy. METHOD: We studied lymphocyte subpopulations and lymphokine production, after 96 h of phytohemagglutinin (PHA) stimulation, from normal and Type I diabetic pregnant women at delivery time and from the respective cord blood. RESULTS: Peripheral blood mononuclear cells (PBMC) from both normal and Type I diabetic mothers showed an increase in CD8+ and a decrease in CD4+ cells compared to the respective cord blood mononuclear cells (CBMC). Moreover, Type I PBMC showed a lower number of “activate…

Th1 and Th2 cell phenotypes in AITD: role of allergy in Graves' disease

Distinct subsets of intrathyroidal regulatory CD4+CD25+ and CD8+CD25+ T cells in Hashimoto's thyroiditis: Potential role in the neoplastic cell growth.

L’espressione di citochine TH1 e TH2 nei pazienti affetti da tireopatia autoimmune (AITD) e diabete di tipo 1

Metabolic parameters and adipokine profile during GH replacement therapy in children with GH deficiency

Objective: GH replacement therapy in children with GH deficiency (GHD) mainly promotes linear growth. Not only have very few studies fully analyzed the metabolic consequences of GH therapy, but also the question as to whether GH may affect adipokine secretion has been insufficiently investigated. Our aim was to study the effects of GH replacement therapy on auxological data, lipid and glycemic profiles, insulin homeostasis (HOMA-IR) and serum adipokines in children. Methods: This was a 1-year prospective study. Thirty-four GHD children (11.6 ± 2.6 years) and thirty healthy matched controls were enrolled. Children affected by GHD were studied both before beginning continuous GH replacement t…

High expression of peroxisome proliferator-activated receptor gamma (PPRgamma) in infiltrating lymphocytes from glands with Hashimoto's thyroiditis.

Changes in lipid profile and adipokine levels in GH deficient children during GH replacement therapy.

Associazione tra variabilità glicemica (ottenuta attraverso il monitoraggio glicemico continuo delle 48h) ed interleukina-6 in soggetti con sindrome metabolica

No effect of GH/IGF-1 excess on adrenal glands in acromegalic patients

Variabilità glicemica da monitoraggio continuo delle 48h ed interleukina-6 in soggetti con sindrome metabolica

Study of T-cell activation in Type I diabetic patients and pre-Type I diabetic subjects by cytometric analysis: Antigen expression defectin vitro

In Type I diabetes the observation of a decreased release of interleukin-2 (IL-2) and soluble IL-2 receptors by means of stimulated lymphocytes in vitro indicates that a primary immunoregulatory defect may be involved. To confirm this hypothesis we investigated the T-cell activation trend, evaluating the surface expression of IL-2 receptor (CD25), transferrin (CD71), HLA class II (DR), and CD69 phenotypes after in vitro stimulation with phytohemagglutinin (PHA; 1 and 10 micrograms/ml) and concanavalin A (12.5 micrograms/ml) in six newly diagnosed Type I diabetics and six islet cell- and insulin autoantibody-positive first-degree relatives. As controls were studied six long-standing Type I d…

Lower insulin sensitivity differentiates hirsute from non-hirsute Sicilian women with polycystic ovary syndrome

Objective: It is well known that hyperandrogenism and insulin-resistance with or without compensatory hyperinsulinism are closely associated, but the Rotterdam Consensus has concluded that principally obese women with polycystic ovary syndrome (PCOS) should be evaluated for the metabolic syndrome. Our aim was to study insulin sensitivity in PCOS women with hirsutism regardless of obesity. Methods: Clinical characteristics, sex hormones and fasting- and after OGTT-glycemia and insulinemia, homeostatic model of insulin resistance (HOMA IR), and Matsuda index of insulin sensitivity were analyzed in 130 women with PCOS. Hirsutism has been evaluated through the Ferriman–Gallwey (FG) map scoring …

Leptina e adiponectina nella valutazione del compenso glicometabolico in corso di diabete mellito di tipo 1 normopeso.

Glycaemic variability from subcutaneous continuous glucose monitoring and interleukin-6 concentrations in subjects with metabolic syndrome.

Effetti acuti del caffè espresso sulla funzione endoteliale e sulla glicemia basale in soggetti sani (ISRCTN85096812)

Rotterdam consensus, NIH and EAS: comparison among the three different diagnostic criteria for the diagnosis of PCOS.

Resistenze intra-renali, spessore mio-intimale carotideo ed interleukina-6 in soggetti con sindrome metabolica

Different Th1/Th2 cytokine expression in Type 1 diabetes mellitus patients (T1DM) alone or associated with autoimmune thyroid disease (AITD)

Il c-peptide come marker principale nella storia naturale del Diabete tipo 1: ruolo della funzione b-cellulare pancreatica nelle complicanze

Decremento delle cellule T regolatorie (Treg) CD4+CD25+FOXP3+ nei linfociti periferici dei pazienti affetti da diabete di tipo 1.

Differential regulation of Fas-mediated apoptosis in both thyrocyte and lymphocyte cellular compartments correlates with opposite phenotypic manifestations of autoimmune thyroid disease.

Several mechanisms are probably involved in determining the evolution of autoimmune thyroid disease (AITD) towards either hypothyroidism and the clinical syndrome known as Hashimoto's thyroiditis (HT) or toward hyperthyroidism and the symptoms of Graves' disease (GD). To gain further insight into such mechanisms we performed an exhaustive comparative analysis of the expression of key molecules regulating cell death (Fas, Fas ligand [FasL], Bcl-2) and apoptosis in both thyrocytes and thyroid infiltrating lymphocytes (TILs) from patients with either GD or HT. GD thyrocytes expressed less Fas/FasL than HT thyrocytes, whereas GD TILs had higher levels of Fas/FasL than HT TILs. GD thyrocytes exp…

Differente pattern di espressione di citochine a fenotipo Th1 e Th2 nei pazienti affetti da diabete di tipo 1 (T1DM) e con associata tireopatia autoimmune (AITD)

Relative Hypoleptinemia in poor controlled patients with Type 1 Diabetes

L’espressione del “peroxisome proliferator-activated gamma” (PPARgamma) nelle tireopatie croniche autoimmuni

La distribuzione corporea del grasso influenza le resistenze intra-renali e lo spessore intima media carotideo nei soggetti con e senza sindrome metabolica

CD4(+)IL-13(+) cells in peripheral blood well correlates with the severity of atopic dermatitis in children.

BACKGROUND In atopic dermatitis (AD) a Th1/Th2 imbalance has been reported, and interleukin (IL)-13 seems to play a pivotal role in the inflammatory network. We tried to assess the correlation between the immunological marker CD4(+)IL-13(+) and the clinical phase of extrinsic AD in children. METHODS Twenty children with AD were studied. Assessed parameters were: clinical severity (SCORAD index), total serum immunoglobulin E (IgE), blood eosinophil count, and percentage of CD4(+)IFNgamma(+), CD4(+)IL-4(+), CD4(+)IL-13(+) T cells. Determinations were carried out in the acute phase and after clinical remission were achieved. Ten nonatopic-matched children served as controls. RESULTS At baselin…