Virulence genotypes of Helicobacter pylori in Palermo, Italy

TYPING OF LEGIONELLA PNEUMOPHILA SEROGROUP 1 BY SBT, MLVA AND SPOLIGOTYPING

Introduction: The current typing method of Legionella pneumophila recommended by the European EWGLI Consortium is a multi locus sequence typing (MLST)-like protocol called sequence-based typing (SBT).This approach is highly portable and widely used to perform epidemiological surveys and outbreak investigation. Recent studies have demonstrated the values of using the multiple-locus variable-number tandem repeat (VNTR) analysis (MLVA) and the diversity of clustered regularly interspaced short palindromic repeat (CRISPR)-based typing technique (Spoligotyping) as genotyping markers. In this study we used these three methods for typing the L. pneumpophila isolates and for investigating the origi…

Profilo patogenetico e sensibilità ai farmaci di stipiti di S.aureus meticillino-resistente SCCMEC tipo IVa isolati in quattro ospedali palermitani

Analisi genotipica di Helicobacter pylori in campioni bioptici

Farmaco-resistenza in stipiti di Helicobacter pylori isolati da pazienti afferenti a strutture ospedaliere della Sicilia centro-occidentale.

Coinfection of Helicobacter pylori and Epstein-Barr virus in patients with gastric disease in Southern Italy

Helicobacter pylori

High prevalence to resistance of clarithromycin in Helicobacter pylori strains isolated in Sicily .

Caratterizzazione molecolare di stipiti di Helicobacter pylori isolati da soggetti con patologia gastrica

Induction of IL-8 production by Helicobacter pylori strains with different cagA genotype and oipA functional status.

HELICOBACTER PYLORI E GENOTIPI DELLE CITOCHINE IL-1B, IL-1RA, IL-10 in pazienti con carcinoma gastrico

Tipizzazione molecolare di stipiti di Legionella pneumophila sierogruppo 1 isolati da campioni clinici ed ambientali

One-year surveillance of methicillin-resistant Staphylococcus aureus in health-care setting, Palermo, Italy

Background: Methicillin-resistant Staphylococcus aureus (MRSA) is an emerging cause of infections outside of health care settings. We carried out a survey to determine the prevalence and characteristics of MRSA isolates (CA-MRSA, HA-MRSA) identified among in- and outpatients by the clinical microbiology laboratories of four general hospitals in Palermo, Italy during the period February-January 2010. Methods: Participating laboratories performed isolation, confirmed methicillin-resistance by their routine method and weekly sent their strains to the coordinating laboratory at the Department of Sciences for Health Promotion “G. D’Alessandro”, University of Palermo, Italy. The isolates were tes…

Detection of CagA EPIYA motifs in H.pylori DNA extracted from recently collected, frozen, or deparaffinized biopsies and clinical samples

CagA is a major virulence factor of Helicobacter pylori that, once injected into the epithelial cells and phosphorylated on specific bacterial tyrosine residues within repeating EPIYA-A,-B,-C, and -D motifs, localizes to the plasma membrane and interacts with a number of intracellular effectors suggested to play an important role in Helicobacter pylori pathogenesis. EPIYA-D (in East Asian CagA) and EPIYA-C motifs (in Western CagA) are the main sites of CagA phosphorylation and the presence both of EPIYA-D or an increasing number of EPIYA-C motifs, rather than the general CagA positivity, has been associated with more severe gastroduodenal disease. With the aim to analyze EPIYA motifs in 24 …

Effetti dell'infezione da HPV sugli esiti della fecondazione in vitro in pazienti sterili: dati preliminari di uno studio prospettico

Caratterizzazione di stipiti di Staphylococcus aureus meticillino-resistenti con SCCmec di tipo IVa isolati in ospedali palermitani

Cephalosporin resistant Escherichia coli from cancer patients in Cairo, Egypt.

Cephalosporin-resistant Escherichia coli has been increasingly reported worldwide. In this study, 32 cephalosporin resistant E. coli isolates identified from cancer patients in Cairo, Egypt in 2009-2010 were analyzed. Twenty-three were of phylogenetic group D, seven A and one each B1 and B2. By rep-PCR 15 phylogroup D isolates were grouped in four clusters, one with sequence type (ST) 405 and three ST68. Seventeen isolates showed single patterns. blaCTX-M-15 and aac(6')-Ib-cr were the most common resistance determinants. blaOXA-48 and blaVIM were also detected. Multidrug resistant E. coli seriously affects healthcare, especially in immunocompromised hosts, such as cancer patients.

Uso combinato di SBT e PFGE quali metodi molecolari per la tipizzazione di stipiti di Legionella pneumophila sierogruppo 1

Isolamento di Mycobacterium bolletii e Mycobacterium porcinum da pazienti immuno-compromessi

Role of environmental and genetic factor interaction in the aging related disease development: The gastric cancer paradigm.

Tipizzazione molecolare di stipiti di Legionella pneumophila sierogruppo 1 isolati a Palermo

Polyclonal non multiresistant Staphylococcus aureus isolates from clinical cases of infection occurring in Palermo, Italy, during a one-year surveillance period.

BACKGROUND: The evolving epidemiology of methicillin resistant Staphylococcus aureus (MRSA) is characterized by the emergence of infections caused by non multiresistant MRSA carrying staphylococcal chromosomal cassette (SCC)mec IV or V in the healthcare settings. A molecular epidemiological analysis of non multiresistant MRSA isolates from four acute general hospitals was performed in Palermo, Italy, during a one year period. METHODS: For the purpose of the study, MRSA isolates were defined as non multiresistant when they were susceptible to at least three classes of non beta-lactam antibiotics. Seventy-five isolates were submitted to antimicrobial susceptibility testing, multilocus sequenc…

Sequence-Based-Typing (SBT) ed Amplified Fragment Lenght Polymorphism (AFLP) quali metodi di tipizzazione molecolare di stipiti di Legionella pneumophila sierogruppo 1

Valuation of Human,B-defensin 2 and 3 in sera of Helicobacter pylori infected patients

In recent years, anti-microbial peptides have emerged as a critical component of host innate defense. These peptides are gene-encoded natural antibiotics expressed by immune and non-immune cell types including epithelia. Human beta defensin are disulphide linked, low molecular weight cationic peptides that are known to be a major components of innate immune defense mechanisms at mucosal surfaces. Their expression level differs between those that are constitutively expressed and those that are induced upon challenge with inflammatory or pathogen-derived stimuli. To date six members of the human-β-defensins (hBD1-6) family have been identified. Helicobacter pylori (H. pylori), a pathogenic bu…

CARATTERIZZAZIONE MOLECOLARE DI STIPITI DI H.PYLORI isolati da soggetti con patologia gastrica

Helicobacter pylori e genotipi delle citochine IL-1β, IL-1Ra, IL-10 in pazienti con carcinoma gastrico

LEVELS OF HBD2 AND HBD3 IN PATIENTS INFECTED AND NON-INFECTED BY HELICOBACTER PYLORI

Introduction: Infection with Helicobacter pylori (H. pylori) evokes inflammatory and immune responses of the host, which most likely determine the clinical outcome of H. pylori infection. In the gastric epithelium, expression of human alpha-defensins, beta-defensins (hBD)-1, -2 and -3 has been detected in vivo. In particularly hBD3 as well hBD2 is known to be induced in gastric epithelial cells infection by the H. pylori and may be involved in the pathogenesis of H pylori-associated gastritis, possibly through its function as immune and inflammatory mediator. However, during prolonged infection, hBD3 was subsequently downregulated by the H. pylori virulence determinant CagA. Materials and M…

Stipiti di Klebsiella pneumoniae resistenti ai carbapenemi circolanti in differenti reparti dell'A.O.U.P. "P. Giaccone" di Palermo

Caratterizzazione molecolare di stipiti di H.pylori isolati a Palermo mediante analisi dei motivi EPIYA di CagA

Induction of IL-8 production by H.pylori strains with different cagA genotype and oipA functional status

Analisi dei motivi EPIYA di CagA in campioni bioptici di soggetti con patologia gastrica H.pylori-correlata

Isolamento e tipizzazione di stipiti di Dipodascus capitatus: patogeno opportunista emergente

L’incidenza delle infezioni micotiche ha subito negli ultimi decenni un incremento rilevante, sia in ambito ospedaliero che comunitario. In ambiente ospedaliero il problema sta assumendo dimensioni preoccupanti poiché, sebbene Candida spp. si debba considerare il principale responsabile di infezioni correlate ai miceti, emergono altre specie il cui ruolo deve essere accertato. In particolare Dipodascus capitatus, forma teleomorfica di Geotrichum capitatum, microrganismo che può risiedere nel suolo, sulla pelle e nel tratto respiratorio e gastroenterico, nel passato considerato un contaminate o un innocuo colonizzatore, da qualche anno è stato associato ad infezioni soprattutto nei pazienti …

Characterization of MRSA from Palermo, Italy, and description of prevalent clones