Soil DNA evidence for altered microbial diversity after long-term application of municipal wastewater

International audience; Water resources constitute a social, agricultural and economic problem in most countries of the southern Mediterranean Basin. Alternative strategies have been developed such as the reuse of municipal wastewater for irrigation in agriculture. Despite numerous advantages for soil fertility and crop productivity, recycling wastewater in soils also has several ecotoxicological and sanitary problems. Few investigations have assessed the risk by evaluating the impact of wastewater irrigation on soil microbes. Here, we report for the first time the short- and long-term effects of treated municipal wastewater irrigation on the density and genetic structure of bacterial and f…