Author: Gurpaul S. Sidhu

0000000000465105

AUTHOR

Gurpaul S. Sidhu

showing 1 related works from this author

Enhanced tissue penetration of antibodies through pressurized immunohistochemistry

2020

ABSTRACTTo address the inefficiency of passive diffusion for antibody penetration in thick tissue samples, which limits clearing-technique applications, we developed a versatile and simple device to perform antibody incubation under increased barometric pressure. Pressurized immunohistochemistry greatly improves the uniformity, intensity, and depth of fluorescent immunostaining in thick human and mouse brain samples. Furthermore, pressurized immunohistochemistry substantially decreases the time required for classic staining of thin sections.SUBMISSION CATEGORYNew Results

biologyChemistrybiology.proteinImmunohistochemistryIncreased barometric pressurePenetration (firestop)AntibodyTissue penetrationImmunostainingBiomedical engineeringStaining

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