0000000000470168

AUTHOR

Gianluigi Cabiddu

showing 2 related works from this author

Proteomic characterization of the mucosal pellicle formed in vitro on a cellular model of oral epithelium

2020

The oral mucosal pellicle is a thin lubricating layer generated by the binding of saliva proteins on epithelial oral cells. The protein composition of this biological structure has been to date studied by targeted analyses of specific salivary proteins. In order to perform a more exhaustive proteome characterization of pellicles, we used TR146 cells expressing or not the transmembrane mucin MUC1 and generated pellicles by incubation with human saliva and washing to remove unbound proteins. A suitable method was established for the in vitro isolation of the mucosal pellicle by "shaving" it from the cells using trypsin. The extracts, the washing solutions and the saliva used to constitute the…

Proteomics0301 basic medicineSalivaTR146/MUC1 cells[SDV]Life Sciences [q-bio]BiophysicsPluncBiochemistryEpithelium03 medical and health sciencesTandem Mass SpectrometrymedicineHumansDental PellicleSalivary Proteins and PeptidesSalivaproteomicMUC1Mucosal pellicle030102 biochemistry & molecular biologyChemistryMucinTrypsinIn vitroTransmembrane proteinCellular model of oral mucosa030104 developmental biologyBiochemistryProteome[SDV.AEN]Life Sciences [q-bio]/Food and Nutritionmedicine.drugJournal of Proteomics
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Proteomic characterization of the mucosal pellicle formed in vitro on a cellular model of oral epithelium

2019

International audience

saliva[SDV.AEN] Life Sciences [q-bio]/Food and Nutritionmucosal pellicle[SPI.GPROC] Engineering Sciences [physics]/Chemical and Process Engineeringcell model[SPI.GPROC]Engineering Sciences [physics]/Chemical and Process Engineering[SDV.AEN]Life Sciences [q-bio]/Food and NutritionmucosaComputingMilieux_MISCELLANEOUS
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