0000000000470737
AUTHOR
Valérie Jérôme
Untersuchung von neuartigen Nanopartikeln für ihre Anwendung in der Biotechnologie
© 2014 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim www.cit-journal.com In der organischen Synthese werden oftmals hohe Drucke und Temperaturen wie auch konzentrierte und schadliche Losungen eingesetzt. Unter allgemein milderen Bedingungen konnen durch biotechnologische Alternativen gleiche Zielprodukte erhalten werden. e-Caprolacton wird industriell als Monomer zur Polymerisation zu Polycaprolacton eingesetzt. Dieses biologisch abbaubare Polymer ist mischbar mit vielen verschiedenen Polymeren, was einen sehr breiten Anwendungsbreich schafft wie beispielsweise fur die Medizin, als Weichmacher oder als Druckmaterial fur 3D-Drucker. Chemisch erfolgt die Synthese von e-Caprolacton durch die Baey…
Co-transfection of star-shaped PDMAEMAs enhance transfection efficiency of protamine/pDNA complexes in the presence of serum
Abstract In previous studies, we have found that un-complexed free cationic polymers greatly promote gene transfection efficiency of pDNA complexes. Dividing the gene transfection system into two parts, bound chains and free chains, we exploited mixing different biomaterials as the second component of our designed system to improve gene transfection outcome. In this study, we investigated the effectiveness of star-shaped poly(2-(dimethylamino)ethyl methacrylate) (PDMAEMA) with different arm numbers as free chains (i.e., co-transfection agent) while using natural salmon protamine as bound chains. We explored the gene transfer ability of the system in both serum free and complete cell culture…
Influence of Polyplex Formation on the Performance of Star-Shaped Polycationic Transfection Agents for Mammalian Cells
Genetic modification (“transfection”) of mammalian cells using non-viral, synthetic agents such as polycations, is still a challenge. Polyplex formation between the DNA and the polycation is a decisive step in such experiments. Star-shaped polycations have been proposed as superior transfection agents, yet have never before been compared side-by-side, e.g., in view of structural effects. Herein four star-shaped polycationic structures, all based on (2-dimethylamino) ethyl methacrylate (DMAEMA) building blocks, were investigated for their potential to deliver DNA to adherent (CHO, L929, HEK-293) and non-adherent (Jurkat, primary human T lymphocytes) mammalian cells. The investigated vectors …
Systematic Study of a Library of PDMAEMA-Based, Superparamagnetic Nano-Stars for the Transfection of CHO-K1 Cells.
The introduction of the DNA into mammalian cells remains a challenge in gene delivery, particularly in vivo. Viral vectors are unmatched in their efficiency for gene delivery, but may trigger immune responses and cause severe side-reactions. Non-viral vectors are much less efficient. Recently, our group has suggested that a star-shaped structure improves and even transforms the gene delivery capability of synthetic polycations. In this contribution, this effect was systematically studied using a library of highly homogeneous, paramagnetic nano-star polycations with varied arm lengths and grafting densities. Gene delivery was conducted in CHO-K1 cells, using a plasmid encoding a green fluore…