0000000000480674
AUTHOR
M. Montesinos
CHANGES IN ALBINO RAT TESTICLE INTERSTITIAL CELLS AFTER PITUITARY STIMULATION IN VIVO WITH HeNe LASER
Population I Leydig cells of the central part of the Albino rat testicle showed signs of increased but nonspecific activity in the entire series of experimental animals compared with sham irradiated controls (n = 30 in each group) after anterior pituitary stimulation with low incident energy levels of the HeNe laser at 632.8 nm with an incident power at the pituitary calculated as 2.75 ± 0.25 mW. The authors conclude that LLLT applied to the pituitary in the rat model at the parameters used in the present study may have an effect on testosterone production. Further quantitative and qualitative work is required to assess the significance of the increase in activity of type I Leydig cells and…
Additional file 1: Figure S1. of Apremilast, a novel phosphodiesterase 4 (PDE4) inhibitor, regulates inflammation through multiple cAMP downstream effectors
Adenosine A2A receptor (A2AR) activation and apremilast do not additively increase responsive element binding protein (cAMP). Raw 264.7 cells were incubated with cumulative concentrations of apremilast (6 nM to 1 μM), apremilast + CGS21680 1μM 15 minutes before apremilast, or cumulative concentrations of CGS21680 alone (6 nM to 1 μM), followed by treatment with lipopolysaccharide (LPS) 1 μM for 20 minutes. Then, intracellular cAMP levels were measured as described under “Materials and methods”. Data represent means ± standard error of the mean of at least three independent experiments. (TIFF 170 kb)
Additional file 2: Figure S2. of Apremilast, a novel phosphodiesterase 4 (PDE4) inhibitor, regulates inflammation through multiple cAMP downstream effectors
Permanent transduction of shRNA for protein kinase (PKA) dramatically decreases PKA protein expression in the Raw 264.7 cell line, as determined by western blotting. (TIFF 268 kb)