0000000000484856
AUTHOR
Tobias L. Roß
Measurement of Protein Synthesis: In Vitro Comparison of 68Ga-DOTA-Puromycin, [3H]Tyrosine, and 2-Fluoro-[3H]tyrosine
Aim: Puromycin has played an important role in our understanding of the eukaryotic ribosome and protein synthesis. It has been known for more than 40 years that this antibiotic is a universal protein synthesis inhibitor that acts as a structural analog of an aminoacyl-transfer RNA (aa-tRNA) in eukaryotic ribosomes. Due to the role of enzymes and their synthesis in situations of need (DNA damage, e.g., after chemo- or radiation therapy), determination of protein synthesis is important for control of antitumor therapy, to enhance long-term survival of tumor patients, and to minimize side-effects of therapy. Multiple attempts to reach this goal have been made through the last decades, mostly u…
PET Chemistry: An Introduction
One major advantage of radioactivity is its extremely high sensitivity of detection. Regarding the medical applicability of radioactivity, it permits non-invasive in vivo detection of radiolabelled compounds at nano- to picomolar levels. The use of substances at such low concentrations usually precludes a physiological, toxic or immunologic response of the investigated biological system. Consequently, the considered physiological process or system is examined in an unswayed situation. Furthermore, a wide range of substances, even those which are toxic at higher concentrations, become considerable for the development of radiopharmaceuticals and use in nuclear medicine. In contrast to the wid…
PET Chemistry: Radiopharmaceuticals
Although many radiolabelled compounds for PET imaging have been developed so far, only a few have reached the status of a clinically established and routinely used PET radiopharmaceutical. At the early stage of development, a reasonable medical indication is obviously fundamental for a PET radiopharmaceutical to be further considered as clinically relevant. However, besides a favourable in vivo behaviour and appropriate imaging characteristics, certain criteria have to be fulfilled, such as a fast, straightforward and reliable radiosynthesis; an assured stability of the label as well as of the compound itself and a good availability of a suitable precursor. In particular, the ease and relia…