0000000000512306

AUTHOR

Peter Güntert

showing 2 related works from this author

NMR Investigation of Structures of G-Protein Coupled Receptor Folding Intermediates

2016

Folding of G-protein coupled receptors (GPCRs) according to the two-stage model (Popot, J. L., and Engelman, D. M. (1990) Biochemistry 29, 4031-4037) is postulated to proceed in 2 steps: partitioning of the polypeptide into the membrane followed by diffusion until native contacts are formed. Herein we investigate conformational preferences of fragments of the yeast Ste2p receptor using NMR. Constructs comprising the first, the first two, and the first three transmembrane (TM) segments, as well as a construct comprising TM1-TM2 covalently linked to TM7 were examined. We observed that the isolated TM1 does not form a stable helix nor does it integrate well into the micelle. TM1 is significant…

0301 basic medicine10120 Department of ChemistryBioquímicaSaccharomyces cerevisiae Proteins1303 BiochemistryProtein ConformationStereochemistrySaccharomyces cerevisiaeBiochemistryMicelleRessonància magnètica nuclear1307 Cell BiologyG03 medical and health sciencesprotein coupled receptorGPCRProtein Domains540 Chemistry1312 Molecular BiologyAmino Acid SequenceNuclear Magnetic Resonance BiomolecularMolecular BiologyMicellesG protein-coupled receptorSequence Homology Amino Acid030102 biochemistry & molecular biologyChemistryProteïnes de membranaFoldingCell BiologyTransloconPeptide FragmentsTransmembrane proteinNMRFolding (chemistry)Crystallography030104 developmental biologyStructural biology10036 Medical ClinicProtein Structure and FoldingReceptors Mating FactorHelixProtein folding
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NMR Exchange Format: a unified and open standard for representation of NMR restraint data

2015

SCOPUS: le.j

0303 health sciencesElectronic Data ProcessingMagnetic Resonance SpectroscopyComputer sciencebusiness.industryeducationRepresentation (systemics)virus diseasesGénéralitésNuclear magnetic resonance spectroscopy010402 general chemistry01 natural sciencesArticle0104 chemical sciencesComputational science03 medical and health sciencesSoftwareNuclear magnetic resonanceStructural BiologyOpen standard[CHIM.ANAL]Chemical Sciences/Analytical chemistrybusinessMolecular BiologySoftware030304 developmental biologyNature structural & molecular biology
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