Morphokinetic analysis and embryonic prediction for blastocyst formation through an integrated time-lapse system.

Objective To describe the events associated with the blastocyst formation and implantation that occur in embryos during preimplantation development based on the largest sample size ever described with time-lapse monitoring. Design Observational, retrospective, single-center clinical study. Setting University-affiliated private IVF center. Patient(s) A total of 7,483 zygotes from 990 first treatments of intracytoplasmic sperm injection (ICSI; 627 of oocyte donor vs. 363 autologous oocyte cycles), of which 832 blastocysts were transferred. Intervention(s) No patient intervention. Embryos were cultured in a time-lapse monitoring system, and the embryos were transferred on day 5 after ICSI. Emb…

Human blastocysts derived from monopronuclear zygotes: a biological model for the study of ploidy, euploidy, topography and heteroparental inheritance

Effect of different 6-dimethylaminopurine (6-DMAP) treatments on reversible arresting of mono- and tripronuclear embryos at the pronuclear stage