Radiosensitizing effect of Curcumin loaded lipid nanoparticles in breast cancer cells

Effect of composition of Solid Lipid Nanoparticles on their chemical-physical properties and potential for gene therapy

Sustained release of silibinin to the posterior segment of the eye by mucoadhesive NLC

Enhanced pulmonary delivery of Salmeterol Xinafoate (SX) by mucoadhesive microparticles

Nanoassemblies Based on Supramolecular Complexes of Nonionic Amphiphilic Cyclodextrin and Sorafenib as Effective Weapons to Kill Human HCC Cells

Sorafenib (Sor), an effective chemiotherapeutic drug utilized against hepatocellular carcinoma (HOC), robustly interacts with nonionic amphiphilic cyclodextrin (aCD, SC6OH), forming, in aqueous solution, supramolecular complexes that behave as building blocks of highly water-dispersible colloidal nanoassemblies. SC6OH/Sor complex has been characterized by complementary spectroscopic techniques, such as UV-vis, steady-state fluorescence and anisotropy, resonance light scattering and H-1 NMR. The spectroscopic evidences and experiments carried out in the presence of an adamantane derivative, which competes with drug for CD cavity, agree with the entrapment of Sor in aCD, pointing out the role…

Biocompatible Lipid Nanoparticles as Carriers to Improve Curcumin Efficacy in Ovarian Cancer Treatment

Curcumin is a natural molecule with proved anticancer efficacy on several human cancer cell lines. However, its clinical application has been limited due to its poor bioavailability. Nanocarrier-based drug delivery approaches could make curcumin dispersible in aqueous media, thus overtaking the limits of its low solubility. The aim of this study was to increase the bioavailability and the antitumoral activity of curcumin, by entrapping it into nanostructured lipid carriers (NLCs). For this purpose here we describe the preparation and characterization of three kinds of curcumin-loaded NLCs. The nanosystems allowed the achievement of a controlled release of curcumin, the amounts of curcumin r…

Lipid nanocarriers containing sorafenib inhibit colonies formation in human hepatocarcinoma cells

Here, the potential of two nanostructured lipid carriers (NLC) for controlled release of sorafenib was evaluated. The obtained systems showed characteristics suitable as drug delivery systems for the treatment of hepatocellular carcinoma (HCC) through parenteral administration. The use of a mixture between a solid lipid (tripalmitin) with a liquid lipid (Captex 355 EP/NF or Miglyol 812) to prepare NLC systems could give a higher drug loading capacity and a longer term stability during storage than that obtained by using only solid lipids. The obtained nanoparticles showed a nanometer size and high negative zeta potential values. Scansion electron microscopy (SEM) of the sorafenib loaded NLC…

Cationic solid lipid nanoparticles complexed with genetic material for liver tumor treatment

Concept Gene therapy is a growing field of medicine with great potential for the treatment of several diseases and it is based on the delivery of nucleic acids (DNA, RNA, etc.,) to specific cells. To achieve their therapeutic effects, the nucleic acids need to cross several biological barriers and be protected from the degradation by nucleases, present in biological fluids and intracellular compartments, to successfully gain access to their intracellular targets. To overcome these hurdles, it is necessary to deliver the genetic material with biocompatible carriers able to facilitate its translocation across the cell membranes and protect it from being degraded while circulating in the blood…

CATIONIC SLN AS TARGETING DRUG DELIVERY SYSTEMS

Nanoscience and nanotechnology have received much attention in the last decade and they actually form one of the most important fields of technology and innovation. Due to rapid progresses in nanotechnology and biotechnology, nanoparticles have come to be seen as a viable vehicle for the delivery and release of drugs and nucleic acids. Within this field, the use of solid lipid nanoparticles (SLNs) is of particular importance. In particular, cationic SLN are promising non-viral gene delivery carriers suitable for systemic and topic administration. The surface of these nanoparticles is positively charged and the negatively charged nucleic acids are complexed to the their surface. The obtained…

DNA PLASMID DELIVERY INTO HEPATOCELLULAR CARCINOMA CELLS BY COMPLEXATION WITH SOLID LIPID NANOPARTICLES

Delivery of shNupr1 plasmid by solid lipid nanoparticles reduces the expression of Nupr1 gene in hepatocellular carcinoma cells

Delivery of shRNA plasmid DNA by cationic solid lipid nanoparticles for gene silencing in human hepatocarcinoma cells

Preparation, characterization and biological evaluation of Nanostructured Lipid Carriers (NLCs) conteining sorafenib

PEGylated graphene oxide (GO-PEG) as new carrier for chemotherapeutic agent delivery

Graphene, a single layer of sp2 -hybridized carbon atoms arranged in a honeycomb two-dimensional (2-D) crystal lattice, has evoked enormous interest throughout the scientific community since its first appearance in 2004. Due to its unique structure and geometry, graphene possesses remarkable physical–chemical properties (including large specific surface area and biocompatibility) that enable it to be an ideal material for several of applications, ranging from quantum physics, nanoelectronics, energy research, catalysis and engineering of nanocomposites and biomaterials. In the area of nanomedicine, graphene and its derivatives can be exploited for a broad range of applications, including a …

Cationic Solid Lipid Nanoparticles (SLN) for shNupr1 plasmid delivery in the treatment of hepatocellular carcinoma (HCC)

ENTRAPMENT OF TYRPHOSTIN AG 14-78 INTO LIPID NANOPARTICLES IMPROVES ITS ANTITUMOR ACTIVITY AGAINST HUMAN HEPATOCARCINOMA CELLS

CATIONIC SLN AS TARGETING GENE DELIVERY SYSTEMS FOR HEPATOCELLULAR CARCINOMA

Hepatocellular carcinoma (HCC) is the sixth most common cancer worldwide and the third leading cause of cancer-related deaths. In the United States, the incidence of HCC has almost tripled during the past two decades and HCC has become one of the fastest growing cancers. While surgical removal of tumor tissues is an effective approach to protect relatively healthy liver tissue, it is only applicable to a small subset of HCC patients with specific pathological conditions, such as confined tumor mass without portal hypertension. Therefore, there is an urgent need to develop novel therapeutic strategies to treat this deadly disease. Systemic tumor-targeted gene delivery is attracting increasin…

Curcumin delivered by solid lipid nanoparticles potentiates radiation effects in MCF7 cancer cells

Effects in cigarette smoke stimulated bronchial epithelial cells of a corticosteroid entrapped into nanostructured lipid carriers

Background Nanomedicine studies have showed a great potential for drug delivery into the lung. In this manuscript nanostructured lipid carriers (NLC) containing Fluticasone propionate (FP) were prepared and their biocompatibility and effects in a human bronchial epithelial cell line (16-HBE) stimulated with cigarette smoke extracts (CSE) were tested. Results Biocompatibility studies showed that the NLC did not induce cell necrosis or apoptosis. Moreover, it was confirmed that CSE increased intracellular ROS production and TLR4 expression in bronchial epithelial cells and that FP-loaded NLC were more effective than free drug in modulating these processes. Finally, the nanoparticles increased…

Surfactant effect on the physicochemical characteristics of cationic solid lipid nanoparticles

Solid lipid nanoparticles (SLNs) may be considered as a new approach for therapeutics for many diseases. In addition to drug delivery, their use as non-viral vectors for gene delivery can be obtained by including cationic lipids, which provide a positive surface potential that favors binding to the nucleic acids as DNA, siRNA, miRNA, etc. In fact, the addition of cationic surfactants is indispensable for obtaining nanoparticles with surface positive charge. In this study, three different cationic lipids (dioctadecyl dimethyl ammonium bromide, cetyltrimethyl ammonium bromide, cetylpyridinium chloride) and Brij 76 as nonionic surfactant were employed to formulate Precirol ATO 5 based cSLN usi…

Synthesis and characterization of PEGylated graphene oxide for sorafenib modified release

Concept Graphene, a single layer of sp2-hybridized carbon atoms arranged in a honeycomb two-dimensional (2-D) crystal lattice, has evoked enormous interest throughout the scientific community since its first appearance in 2004. Due to its unique structure and geometry, graphene possesses remarkable physical-chemical properties (including large specific surface area and biocompatibility) that enable it to be an ideal material for several applications, ranging from quantum physics, nanoelectronics, energy research, catalysis and engineering of nanocomposites and biomaterials. In the area of nanomedicine, graphene and its derivatives can be exploited for a broad range of applications, includin…

Effects of Homogenization Technique and amount of Surfactants on Physical-Chemical Properties of Nanostructured Lipid Carriers (NLCs).

Encapsulation of sorafenib into lipid nanoparticles improves its antitumor activity.

Effects of kind and amount of surfactants on physical-chemical properties of cationic solid lipid nanoparticles (SLN) for the treatment of hepatocellular carcinoma.

Lipid nanocarrier for the treatment of hepatocellular carcinoma

Hepatocellular carcinoma (HCC) is the sixth most prevalent cancer and the third most frequent cause of cancer-related death. Unlike most solid tumors the incidence and mortality of HCC have increased in the United States and Europe in the past decade. Most patients are diagnosed at advanced stages, so there is an urgent need for new systemic therapies [1,2]. To improve the delivery of therapeutic agents to tumor cells in vivo it’s necessary to overcome several problems, such as drug resistance at the tumor level due to physiological barriers (non cellular based mechanism), drug resistance at the cellular level (cellular mechanism), drug distribution, biotransformation and clearance of antic…