0000000000537901

AUTHOR

Javier Alonso-del-real

0000-0002-6474-9040

showing 6 related works from this author

Dominance of wine Saccharomyces cerevisiae strains over S. kudriavzevii in industrial fermentation competitions is related to an acceleration of nutr…

2019

Grape must is a sugar‐rich habitat for a complex microbiota which is replaced by Saccharomyces cerevisiae strains during the first fermentation stages. Interest on yeast competitive interactions has recently been propelled due to the use of alternative yeasts in the wine industry to respond to new market demands. The main issue resides in the persistence of these yeasts due to the specific competitive activity of S. cerevisiae. To gather deeper knowledge of the molecular mechanisms involved, we performed a comparative transcriptomic analysis during fermentation carried out by a wine S. cerevisiae strain and a strain representative of the cryophilic S. kudriavzevii, which exhibits high genet…

Grape juicemedia_common.quotation_subjectAdaptive evolutionSaccharomyces cerevisiaeWineIndustrial fermentationSaccharomyces cerevisiaeMicrobiologyYeast populationsCompetition (biology)Saccharomyces03 medical and health sciencesMessenger-RNAMechanismsVitisGene-expressionFood scienceAdaptationEcological interactionsEcology Evolution Behavior and Systematics030304 developmental biologymedia_commonWine0303 health sciencesbiology030306 microbiologyProteinStrain (biology)food and beveragesNutrientsbiology.organism_classificationAdaptation PhysiologicalYeastPhenotypeFermentationFermentationAdaptationPopulation genomicsEnvironmental Microbiology
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New Trends in the Uses of Yeasts in Oenology

2018

Abstract The most important factor in winemaking is the quality of the final product and the new trends in oenology are dictated by wine consumers and producers. Traditionally the red wine is the most consumed and more popular; however, in the last times, the wine companies try to attract other groups of populations, especially young people and women that prefer sweet, whites or rose wines, very fruity and with low alcohol content. Besides the new trends in consumer preferences, there are also increased concerns on the effects of alcohol consumption on health and the effects of global climate change on grape ripening and wine composition producing wines with high alcohol content. Although S…

0301 basic medicineWinedigestive oral and skin physiology030106 microbiologyfood and beveragesYeast03 medical and health sciencesSaccharomyces speciesHigh alcoholFood scienceBusinessAlcohol consumptionWinemakingWine industryOenology
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The Use of Mixed Populations of Saccharomyces cerevisiae and S. kudriavzevii to Reduce Ethanol Content in Wine: Limited Aeration, Inoculum Proportion…

2017

Saccharomyces cerevisiae is the most widespread microorganism responsible for wine alcoholic fermentation. Nevertheless, the wine industry is currently facing new challenges, some of them associate with climate change, which have a negative effect on ethanol content and wine quality. Numerous and varied strategies have been carried out to overcome these concerns. From a biotechnological point of view, the use of alternative non-Saccharomyces yeasts, yielding lower ethanol concentrations and sometimes giving rise to new and interesting aroma, is one of the trendiest approaches. However, S. cerevisiae usually outcompetes other Saccharomyces species due to its better adaptation to the fermenta…

0301 basic medicineMicrobiology (medical)Saccharomyces yeastStarter culturesMicroorganism030106 microbiologylcsh:QR1-502ethanol reductionBiologyEthanol fermentationAliments MicrobiologiaMicrobiologylcsh:Microbiology03 medical and health sciencesWine fermentationFermentation oxygenationFermentacióFood scienceAromaFermentation in winemakingWineEthanol reductionbusiness.industrystarter culturesfood and beveragesbiology.organism_classificationfermentation oxygenationBiotechnologyYeast in winemaking030104 developmental biologywine fermentationViniculturaFermentationAerationbusinessFrontiers in Microbiology
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Effect of temperature on the prevalence of Saccharomyces non-cerevisiae species against a S. cerevisiae wine strain in wine fermentation: competition…

2017

Saccharomyces cerevisiae is the main microorganism responsible for the fermentation of wine. Nevertheless, in the last years wineries are facing new challenges due to current market demands and climate change effects on the wine quality. New yeast starters formed by non-conventional Saccharomyces species (such as S. uvarum or S. kudriavzevii) or their hybrids (S. cerevisiae x S. uvarum and S. cerevisiae x S. kudriavzevii) can contribute to solve some of these challenges. They exhibit good fermentative capabilities at low temperatures, producing wines with lower alcohol and higher glycerol amounts. However, S. cerevisiae can competitively displace other yeast species from wine fermentations,…

0301 basic medicineMicrobiology (medical)030106 microbiologySaccharomyces cerevisiaeSaccharomyces speciesMicrobiologyParadoxusSaccharomycesAliments Microbiologia03 medical and health sciencesBotanyWine fermentationFitnessFermentacióFood scienceWineFermentation in winemakingTemperaturesbiologyCompetitionWine compositiontemperaturefood and beveragesbiology.organism_classificationYeastYeast in winemakingViniculturaFermentation
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Lipid Composition Analysis Reveals Mechanisms of Ethanol Tolerance in the Model YeastSaccharomyces cerevisiae

2021

Saccharomyces cerevisiae is an important unicellular yeast species within the biotechnological and the food and beverage industries. A significant application of this species is the production of ethanol, where concentrations are limited by cellular toxicity, often at the level of the cell membrane. Here, we characterize 61 S. cerevisiae strains for ethanol tolerance and further analyze five representatives with various ethanol tolerances. The most tolerant strain, AJ4, was dominant in coculture at 0 and 10% ethanol. Unexpectedly, although it does not have the highest noninhibitory concentration or MIC, MY29 was the dominant strain in coculture at 6% ethanol, which may be linked to differen…

Phosphatidylethanolamine0303 health sciencesEthanolEcologybiology030306 microbiologyChemistrySaccharomyces cerevisiaeLipidomebiology.organism_classificationApplied Microbiology and BiotechnologySaccharomycesYeastCell membrane03 medical and health scienceschemistry.chemical_compoundmedicine.anatomical_structureMembranemedicineFood science030304 developmental biologyFood ScienceBiotechnologyApplied and Environmental Microbiology
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Adaptive response to wine selective pressures shapes the genome of a Saccharomyces interspecies hybrid

2021

During industrial processes, yeasts are exposed to harsh conditions, which eventually lead to adaptation of the strains. In the laboratory, it is possible to use experimental evolution to link the evolutionary biology response to these adaptation pressures for the industrial improvement of a specific yeast strain. In this work, we aimed to study the adaptation of a wine industrial yeast in stress conditions of the high ethanol concentrations present in stopped fermentations and secondary fermentations in the processes of champagne production. We used a commercial Saccharomyces cerevisiae × S. uvarum hybrid and assessed its adaptation in a modified synthetic must (M-SM) containing high ethan…

GeneticsFermentation in winemakingWine0303 health sciencesExperimental evolutionStrain (chemistry)030306 microbiologySaccharomyces cerevisiaeGeneral MedicineSaccharomyces cerevisiaeBiologybiology.organism_classificationGenome sequencingSaccharomycesSaccharomyces uvarumYeast03 medical and health sciencesS. uvarumArtificial hybridAdaptationAdaptationRNA-seq030304 developmental biology
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