Genetic manipulation of spiramycin-producing strain Streptomyces ambofaciens ATCC 23877 by φC31 Att/Int system-based vectors results in a significant…

The genus Streptomyces produces about two-thirds of naturally occurring antibiotics and many other biologically active secondary metabolites. The genetic manipulation of Streptomycetes is often labor and time intensive due to their large genome and complex development. Much progress has been made to develop gene transfer methods useful to construct antibiotic-producing strains with improved properties. The φC31 Att/Int system is an integration system that has been widely used to produce stable recombinants in Actinomycetes and its integration site is an attB site located in a pirin-like gene (pirA) of the bacterial chromosome. In this study we demonstrate that the integration of φC31 DNA in…

Modello di percorso diagnostico e terapeutico per l'arteriopatia obliterante periferica

2012

FATTORI PSICOLOGICI DI INFLUENZA SULL’ADHERENCE AL TRATTAMENTO E SULLA QUALITÀ DELLA VITA IN PAZIENTI CON VASCULOPATIE. RISULTATI PRELIMINARI

2012

Lo studio della Qualità della Vita (QdV), dell’assetto psicologico e dell’adherence alle prescrizioni mediche in pazienti affetti da disturbi vascolari è un obiettivo indispensabile, sia per la valutazione degli outcome clinici che per la scelta di trattamenti medici adeguati (Piscione et al., 2009). In accordo con la letteratura sulle malattie croniche, intendiamo valutare il peso esercitato da ansia, depressione, strategie di coping, percezione di malattia e alessitimia sullo stato di salute e sulla QdV di pazienti con vasculopatie. METODO Il disegno di ricerca prevede la somministrazione dei seguenti questionari: The Hospital Anxiety and Depression Scale (HADS) (Zigmond e Snaith, 1983); …

MASS-PRODUCED ELEMENT IN STRUCTURAL GLASS: “ARTIFICIAL VINEYARD”

2014

The aim is to design an architectural element flexible and easily adaptable to different urban situations using a material such as glass that, for the last years, is being employed as a structural element. The mass-production is rarely applied to glass construction systems that instead are usually designed case by case. The paper reports the results of a research carried out by the Dipartmento di Architettura and Dipartimento DICAM (Dipartimento di Ingegneria Civile, Ambientale, Aerospaziale, dei Materiali), of the University of Palermo, within the project "VESTRA" (POFESR 2007/2013 - Action Line 4.1.1.1), that aims to the experimentation of mass-produced elements in structural glass. The p…

Software for the heat flow evaluation of the nearly-zero houses

2015

The windows in a building are the weakest part for energy saving and reduced consumption. In particular, the choice of window frames and glazing systems directly influences the amount of heat loss through a houses envelope. The paper will show the results of a work developed by PADesign srl company (Accademic Spin off of the Università degli Studi di Palermo). This is a software tool that allows a rapid comparison about the windows thermal performances, by calculating the global thermal transmittance and by showing the trend of the heat flow. The tool has a simple graphical interface that allows the users to select the climate data, the window typologies, the geometric features and the mate…

GLOBAL SAVING EVALUATION THROUGH A RETROFITTING ACTION

2014

The paper shows a work that was carried out by the authors as members of the PADesign Company, academic spin off of University of Palermo. Currently, energy retrofit of existing building has become an important goal, because it is now widely recognized that the buildings are one of the main causes of CO2 production and of non-renewable energy consumptions. To act on the energy performance of existing buildings (adapting their performances to those required by regulations) is an important strategy to reduce energy consumptions since they are much more than new buildings. Through the application of innovative technologies and methodologies and the choice of appropriate investments, the retrof…