Use of TaqMan® real-time PCR for rapid detection of Salmonella enterica serovar Typhi
We evaluated the performances of a newly designed real-time polymerase chain reaction (PCR) assay using TaqMan® probes to detectSalmonellaTyphi. TaqMan® real-time PCR assays were performed by designed primers and probe based on thestaGgene for detectingS.Typhi. The specificity of the assay was evaluated on 15Salmonellaserovars. The analytical specificity was evaluated on 20 non-Salmonellamicroorganisms. The analytical sensitivity was assessed using decreasing DNA quantities ofS.Typhi ATCC 19430. Finally the detection capability of the TaqMan® real-time PCR assay on isolates recovered from patients withSalmonellainfections was compared to the conventional PCR assay. OnlyS.Typhi strain had po…
Genetic relatedness among isolates of Shigella sonnei carrying class 2 integrons in Tehran, Iran, 2002–2003
Abstract Background Shigella spp. are major cause of diarrhoeal disease in both developing and developed countries. Shigella sonnei is the serogroup of Shigella most frequently responsible for sporadic and epidemic enteritis in developed countries. In recent years the emergence and spread of S. sonnei biotype g carrying class 2 integron have been frequently reported in many countries. Recently, S. sonnei has been reported as the prevalent serogroup of Shigella in Iran. The present study was carried out to investigate phenotypic and genetic characteristics of Shigella sonnei isolates identified in the years 2002 and 2003 in Tehran, Iran. Methods Biotyping, drug susceptibility testing, pulsed…