0000000000583319
AUTHOR
M. Scholl
Model network architectures in vitro on extracellular recording systems using microcontact printing
A PDMS stamp is used to transfer a synthetic peptide in a given pattern to any suitable surface. Using this method two-dimensional neuronal model networks could be formed on glass substrates as well as on electronic devices and adjusted to the given microelectronic structure. The present work focuses on the mechanism of neurite guidance under simplified in vitro conditions, using in vitro guidance cues and outline the incorporation of these interfacial methods into microelectronic sensor devices.
Ordered networks of rat hippocampal neurons attached to silicon oxide surfaces.
The control of neuronal cell position and outgrowth is of fundamental interest in the development of applications ranging from cellular biosensors to tissue engineering. We have produced rectangular networks of functional rat hippocampal neurons on silicon oxide surfaces. Attachment and network formation of neurons was guided by a geometrical grid pattern of the adhesion peptide PA22-2 which matches in sequence a part of the A-chain of laminin. PA22-2 was applied by contact printing onto the functionalised silicon oxide surface and was immobilised by hetero-bifunctional cross-linking with sulfo-GMBS. Geometric pattern matching was achieved by microcontact printing using a polydimethylsiloxa…
Extended gate electrode arrays for extracellular signal recordings
Abstract We have fabricated arrays of planar gold electrodes arranged in a matrix of 8×8 with active areas ranging from 6 to 30 μm in diameter. An electronic amplification circuitry based on commercial junction field-effect transistors was used where the gold sensor fields act as extended gate electrodes (EGE) of the transistors, which leads to a new approach for long-term extracellular recording systems in vitro. The high input resistance of the amplification circuitry allows the use of small planar bare gold electrodes without further modification which therefore extends the frequency range of the measuring set-up down to the DC-level. The performance of our recording system has been test…
Control of endothelial cell adhesion to polymer surface by ion implantation
The bio-compatibility of ion implanted polymers has been studied by means of in vitro attachment measurements of bovine aorta endothelial cells. The specimens used were polystyrene (PS), polyethylene (PE), polypropylene (PP) and expanded polytetrafluoroethylene (ePTFE). He+ and Ne+ ion implantation were performed at an energy of 150 keV with fluences between 1 × 1013 to 1 × 1017ions/cm2 at room temperature. Wettability was estimated by means of a sessile drop method. The chemical and physical structures of ion implanted polymers were investigated by contact angle measurements, atomic force microscopy and X-ray photoelectron spectroscopic analysis in relation to cell attachment behavior. The…