0000000000584296

AUTHOR

S. Minafra

showing 9 related works from this author

Decorin transfection induces proteomic modulation and cytoskeletal rioganization in breast cancer cells (8701-BC)

2003

Decorin proteomics
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LARGE-SCALE COMPARATIVE PROTEOMICS OF BREAST CANCER SURGICAL TISSUES

2009

Proteomics BREAST CANCERSettore BIO/06 - Anatomia Comparata E Citologia
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Gene ontology-based annotation and comparative analysis of proteins extracted from proteomics of 100 breast cancer patients.

2009

Background: Current clinical parameters for breast cancer diagnosis and therapy are: tumour size, axillary lymph node status, histological grading and presence or absence of metastases. Prognostic/predictive properties, such as oestrogen and progesterone receptor status, and human epidermal growth factor receptor (HER-2/neu) status are currently used for therapeutic decision. Conversely, it is now emerging that the number of genetic mutations and epigenetic deregulations in cancer is far more higher than previously thought. Therefore, proteomic screening for differential protein expression in subsets of tumor samples is an essential tool for generating data bases and biomarker discovery. Th…

Bioinformatics Resourcesbreast cancer patientSettore BIO/06 - Anatomia Comparata E Citologiaproteomic
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NEW PROTEOMIC EVIDENCE ON DECORIN EFFECTS ON BREAST CANCER CELLS

2015

The estabilishment of a dinamic crosstyalk between the malignant cells and several components of the ECM is a crucial step of the tumor progression. The aim of the present study was to improve the knowledge about the effects of ectopic decorin on the breast cancer cells, starting from our previous proteomic studies. The new proteomic evidences strenghteh the anti-oncogenic effects of decorin and hilight the attention on the decreased expression of the majority of the members of three protein classes closely related to the malignant phenotype: the metabolic enzymes, the S100 family and the cell motility proteins

DECORIN PROTEOMICS
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DECORIN EFFECTS ON PROTEOMIC PROFILING OF BREAST CANCER CELLS: AN UPDATED STUDY

2015

The malignant carcinomas are characterized by several capabilities acquired by the neoplastic cells, among which the ability to invade the extracellular matrix (ECM) and to establish a crosstalk with several ECM components. Under this respect, the extracellular microenvironment is an entity extraordinarily rich of information with opposite signals. Our group has long undertaken the study of the effects of ECM molecules on the behavior of cancer cells in vitro. Among the studied molecules, the decorin was found to exert a non-permissive effect on the growth and motility of the transfected tumor cells. The decorin, belongs to the family of small leucine-rich proteoglycans (SLRP) and is involv…

The malignant carcinomas are characterized by several capabilities acquired by the neoplastic cells among which the ability to invade the extracellular matrix (ECM) and to establish a crosstalk with several ECM components. Under this respect the extracellular microenvironment is an entity extraordinarily rich of information with opposite signals. Our group has long undertaken the study of the effects of ECM molecules on the behavior of cancer cells in vitro. Among the studied molecules the decorin was found to exert a non-permissive effect on the growth and motility of the transfected tumor cells. The decorin belongs to the family of small leucine-rich proteoglycans (SLRP) and is involved physiologically in the fibrillogenesis of collagen. In the last few year a new anti-oncogenic role has been proposed for decorin1. This study aimed to implement the knowledge on the effects of ectopic decorin on breast cancer cells using as a reference point the results already achieved by our research group2 on the experimental model format. By breast cancer cell line 8701-BC and its transfected clone DEC-C2. The extension of the proteomic analysis combined with the mass spectrometry allowed to triplicate the number of identified proteins in our model. Among the newly identified proteins were members of the classes of metabolic enzymes S100 family and cell motility proteins which revealed a net decrease in the decorin transfected cells. Of considerable importance is the observation that these classes of proteins are the most involved in metastatic progression. These results confirm and reinforce the anti-oncogenic role hypothesized for decorin. The work was co-funded by the Italian 5x1000 to COBS.DECORIN
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BEHAVIOVRAL CHANGES OF BREAST CANCER CELLS IN VITRO

2011

BREAST CANCER
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Decorin transfection induces proteomic and phenotypic modulation in breast cancer cells 8701-BC

2008

Decorin is a prototype member of the small leucine-rich proteoglycan family widely distributed in the extracellular matrices of many connective tissues, where it has been shown to play multiple important roles in the matrix assembly process, as well as in some cellular activities. A major interest for decorin function concerns its role in tumorigenesis, as growth-inhibitor of different neoplastic cells, and potential antimetastatic agent. The aim of our research was to investigate wide-ranged effects of transgenic decorin on breast cancer cells. To this purpose we utilized the well-characterized 8701-BC cell line, isolated from a ductal infiltrating carcinoma of the breast, and two derived …

DecorinTransgeneBlotting WesternOligonucleotidesBreast NeoplasmsBiologymedicine.disease_causeProteomicsBiochemistryproteomicsRheumatologyCell Line TumorSettore BIO/10 - BiochimicaCell AdhesionmedicineHumansElectrophoresis Gel Two-DimensionalOrthopedics and Sports MedicineSettore BIO/06 - Anatomia Comparata E CitologiaMolecular BiologyCell ProliferationdecorinExtracellular Matrix ProteinsCell growthGene Expression ProfilingCell BiologyTransfectionbrest cancer cellGene Expression Regulation Neoplasticcarbohydrates (lipids)Settore BIO/18 - GeneticaProteoglycanCell cultureMicroscopy Electron Scanningbiology.proteinCancer researchdecorin; brest cancer cells; proteomicsFemaleProteoglycansCarcinogenesis
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Large-scale comparative proteomics of breast surgical tissues

2009

Breast cancer Clusterization
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Permissive and restrictive influences from breast cancer stroma

2010

The turn-over of extracellular matrix is a physiological process, that in normal conditions and in wound healing respond to spatial and temporal regulatory mechanisms, involving several cell-matrix interaction pathways. Profound changes occur both at cellular and extracellular level, during the progression of various forms of invasive carcinomas. Collagen alterations and cellular effects. The ultrastructural and biochemical analyses of the collagenous stroma of invasive ductal breast carcinoma have demonstrated the occurrence of extensive fragmentation of pre-existing collagen fibrils and new deposition of thinner fibrils formed mostly by 1(I)3 homotrimer collagen of type I [1-3], which app…

breast cancer stroma
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