0000000000586202

AUTHOR

Jie Pan

showing 3 related works from this author

Excitation-Wavelength-Dependent Photocycle Initiation Dynamics Resolve Heterogeneity in the Photoactive Yellow Protein from Halorhodospira halophila

2018

Photoactive yellow proteins (PYPs) make up a diverse class of blue-light-absorbing bacterial photoreceptors. Electronic excitation of the p-coumaric acid chromophore covalently bound within PYP results in triphasic quenching kinetics; however, the molecular basis of this behavior remains unresolved. Here we explore this question by examining the excitation-wavelength dependence of the photodynamics of the PYP from Halorhodospira halophila via a combined experimental and computational approach. The fluorescence quantum yield, steady-state fluorescence emission maximum, and cryotrapping spectra are demonstrated to depend on excitation wavelength. We also compare the femtosecond photodynamics …

LightKineticsQuantum yieldMolecular Dynamics Simulation010402 general chemistryPhotochemistryPhotoreceptors Microbial01 natural sciencesBiochemistry/dk/atira/pure/sustainabledevelopmentgoals/life_below_waterStructure-Activity RelationshipBacterial Proteins0103 physical sciencesSDG 14 - Life Below Waterta116Photoactive Yellow ProteinsQuenching (fluorescence)ta114010304 chemical physicsChemistryHalorhodospira halophilaHydrogen BondingChromophoreFluorescence0104 chemical sciencesHalorhodospira halophilaFemtosecondExcitationBiochemistry
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Study of light backgrounds from relativistic electrons in air light-guides

2018

The MOLLER experiment proposed at the Thomas Jefferson National Accelerator Facility plans a precision low energy determination of the weak mixing angle via the measurement of the parity-violating asymmetry in the scattering of high energy longitudinally polarized electrons from electrons bound in a liquid hydrogen target (M{\o}ller scattering). A relative measure of the scattering rate is planned to be obtained by intercepting the M{\o}ller scattered electrons with a circular array of thin fused silica tiles attached to air light guides, which facilitate the transport of Cherenkov photons generated within the tiles to photomultiplier tubes (PMTs). The scattered flux will also pass through …

Nuclear and High Energy PhysicsPhotomultiplierPhysics - Instrumentation and DetectorsPhysics::Instrumentation and DetectorsCherenkov detectorFOS: Physical sciencesElectron01 natural sciencesHigh Energy Physics - Experimentlaw.inventionNuclear physicsHigh Energy Physics - Experiment (hep-ex)Opticslaw0103 physical sciencesNuclear Experiment (nucl-ex)Møller scattering010306 general physicsNuclear ExperimentInstrumentationCherenkov radiationPhysicsScintillation010308 nuclear & particles physicsbusiness.industryScatteringInstrumentation and Detectors (physics.ins-det)Cathode raybusinessNuclear Instruments and Methods in Physics Research Section A: Accelerators, Spectrometers, Detectors and Associated Equipment
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Excitation-Wavelength Dependent Photocycle Initiation Dynamics Resolve Heterogeneity in the Photoactive Yellow Protein from Halorhodospira halophila

2018

Photoactive yellow proteins (PYPs) make up a diverse class of blue-light-absorbing bacterial photoreceptors. Electronic excitation of the p-coumaric acid chromophore covalently bound within PYP results in triphasic quenching kinetics; however, the molecular basis of this behavior remains unresolved. Here we explore this question by examining the excitation-wavelength dependence of the photodynamics of the PYP from Halorhodospira halophila via a combined experimental and computational approach. The fluorescence quantum yield, steady-state fluorescence emission maximum, and cryotrapping spectra are demonstrated to depend on excitation wavelength. We also compare the femtosecond photodynamics …

Photoactive Yellow Proteinsfluoresenssivalokemiaproteiinit
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