0000000000589317

AUTHOR

Kornelia Smalla

showing 3 related works from this author

Long-term organic and mineral fertilization strategies shape the rhizosphere microbiota and performance of lettuce

2019

International audience; Belowground plant-microbe interactions are crucial for plant development and health. Although previous studies have shown that soil microbial communities are influenced by fertilization strategies, less is known about the aboveground plant response to the rhizosphere microbiota assemblage shaped by agricultural management strategies. In our study, we aimed to investigate the effects of long-term fertilization strategies across field sites on the rhizosphere prokaryotic (Bacteria and Archaea) community composition and plant performance. We conducted growth chamber experiments with lettuce (Lactuca sativa L.) cultivated in soils from two long-term field experiments sit…

[SDE] Environmental Sciences[SDE]Environmental Sciencesfood and beverages
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Inter-laboratory evaluation of the ISO standard 11063 "Soil quality - Method to directly extract DNA from soil samples"

2011

International audience; Extracting DNA directly from micro-organisms living in soil is a crucial step for the molecular analysis of soil microbial communities. However, the use of a plethora of different soil DNA extraction protocols, each with its own bias, makes accurate data comparison difficult. To overcome this problem, a method for soil DNA extraction was proposed to the International Organization for Standardization (ISO) in 2006. This method was evaluated by 13 independent European laboratories actively participating in national and international ring tests. The reproducibility of the standardized method for molecular analyses was evaluated by comparing the amount of DNA extracted, …

Microbiology (medical)DNA BacterialMicrobiological TechniquesStandardizationSoil testRibosomal Intergenic Spacer analysis[ SDV.TOX.ECO ] Life Sciences [q-bio]/Toxicology/EcotoxicologyBiologyMicrobiologyDNA Ribosomal[ SDE ] Environmental Sciences03 medical and health sciencesRNA Ribosomal 16SMolecular BiologySoil Microbiology030304 developmental biology2. Zero hungerProtocol (science)0303 health sciences030306 microbiologyEcologybusiness.industryDNA FINGERPRINTReproducibility of ResultsDNAInter-laboratory assay15. Life on landSoil DNA extraction; Standardization; Inter-laboratory assaySoil qualityDNA FingerprintingStandardizationBiotechnologyBacterial Typing TechniquesQPCRDNA profilingSoil water[SDE]Environmental Sciencessoil DNA extraction ; standardization ; inter-laboratory assay ; DNA fingerprint ; qPCR[SDV.TOX.ECO]Life Sciences [q-bio]/Toxicology/EcotoxicologybusinessSoil DNA extractionSoil microbiology
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Inter-laboratory evaluation of the ISO standard 11063 "Soil quality -- Method to directly extract DNA from soil samples"

2011

International audience; Extracting DNA directly from micro-organisms living in soil is a crucial step for the molecular analysis of soil microbial communities. However, the use of a plethora of different soil DNA extraction protocols, each with its own bias, makes accurate data comparison difficult. To overcome this problem, a method for soil DNA extraction was proposed to the International Organization for Standardization (ISO) in 2006. This method was evaluated by 13 independent European laboratories actively participating in national and international ring tests. The reproducibility of the standardized method for molecular analyses was evaluated by comparing the amount of DNA extracted, …

[ SDE ] Environmental SciencesDNA fingerprint[SDE] Environmental SciencesqPCR[SDE]Environmental SciencesInter-laboratory assaySoil DNA extractionStandardization
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