0000000000589375

AUTHOR

Karine Laval

showing 3 related works from this author

Étude comparée du rôle des exsudats racinaires de pois (Pisum sativum) et de féverole (Vicia faba) durant les premières phases d’infection par Aphano…

2018

International audience

[SDV] Life Sciences [q-bio][SDV]Life Sciences [q-bio][SDV.BV.PEP] Life Sciences [q-bio]/Vegetal Biology/Phytopathology and phytopharmacyComputingMilieux_MISCELLANEOUS[SDV.BV.PEP]Life Sciences [q-bio]/Vegetal Biology/Phytopathology and phytopharmacy
researchProduct

Inter-laboratory evaluation of the ISO standard 11063 "Soil quality - Method to directly extract DNA from soil samples"

2011

International audience; Extracting DNA directly from micro-organisms living in soil is a crucial step for the molecular analysis of soil microbial communities. However, the use of a plethora of different soil DNA extraction protocols, each with its own bias, makes accurate data comparison difficult. To overcome this problem, a method for soil DNA extraction was proposed to the International Organization for Standardization (ISO) in 2006. This method was evaluated by 13 independent European laboratories actively participating in national and international ring tests. The reproducibility of the standardized method for molecular analyses was evaluated by comparing the amount of DNA extracted, …

Microbiology (medical)DNA BacterialMicrobiological TechniquesStandardizationSoil testRibosomal Intergenic Spacer analysis[ SDV.TOX.ECO ] Life Sciences [q-bio]/Toxicology/EcotoxicologyBiologyMicrobiologyDNA Ribosomal[ SDE ] Environmental Sciences03 medical and health sciencesRNA Ribosomal 16SMolecular BiologySoil Microbiology030304 developmental biology2. Zero hungerProtocol (science)0303 health sciences030306 microbiologyEcologybusiness.industryDNA FINGERPRINTReproducibility of ResultsDNAInter-laboratory assay15. Life on landSoil DNA extraction; Standardization; Inter-laboratory assaySoil qualityDNA FingerprintingStandardizationBiotechnologyBacterial Typing TechniquesQPCRDNA profilingSoil water[SDE]Environmental Sciencessoil DNA extraction ; standardization ; inter-laboratory assay ; DNA fingerprint ; qPCR[SDV.TOX.ECO]Life Sciences [q-bio]/Toxicology/EcotoxicologybusinessSoil DNA extractionSoil microbiology
researchProduct

Inter-laboratory evaluation of the ISO standard 11063 "Soil quality -- Method to directly extract DNA from soil samples"

2011

International audience; Extracting DNA directly from micro-organisms living in soil is a crucial step for the molecular analysis of soil microbial communities. However, the use of a plethora of different soil DNA extraction protocols, each with its own bias, makes accurate data comparison difficult. To overcome this problem, a method for soil DNA extraction was proposed to the International Organization for Standardization (ISO) in 2006. This method was evaluated by 13 independent European laboratories actively participating in national and international ring tests. The reproducibility of the standardized method for molecular analyses was evaluated by comparing the amount of DNA extracted, …

[ SDE ] Environmental SciencesDNA fingerprint[SDE] Environmental SciencesqPCR[SDE]Environmental SciencesInter-laboratory assaySoil DNA extractionStandardization
researchProduct