0000000000591082

AUTHOR

H Hoehn

showing 2 related works from this author

CD8+CD45RA+CD27-CD28-T-cell subset in PBL of cervical cancer patients representing CD8+T-cells being able to recognize cervical cancer associated ant…

2003

Objective In response to antigenic stimulation, naive MHC-class I restricted and antigen-specific CD8+CD45RA+CD28+T-cells undergo clonal expansion and differentiate into CD8+CD45RO+ memory T-cells. Upon re- encounter with the nominal antigen, CD45RO+ T-cells are able to convert to CD8+CD45RA+CD28-T-cells displaying potent immune effector functions, including TNF-alpha production. This T-cell subpopulation constitutes a minor population in healthy individuals. In the present study we are currently evaluating whether this particular T-cell subset in PBL represents CD8+T-cells which may be able to recognize cervical cancer associated antigens provided by HPV 16 E7. Material and methods Flow-cy…

PopulationUterine Cervical Neoplasmschemical and pharmacologic phenomenaCD8-Positive T-LymphocytesBiologyEpitopeImmune systemCD28 AntigensAntigenAntigens CDT-Lymphocyte SubsetsmedicineHumansCytotoxic T cellAmino Acid SequenceeducationAntigens ViralPapillomaviridaeNeoplasm Stagingeducation.field_of_studyHistocompatibility TestingObstetrics and GynecologyCD28Cancerhemic and immune systemsmedicine.diseasePeptide FragmentsTumor Necrosis Factor Receptor Superfamily Member 7Lymphatic MetastasisImmunologyCytokinesLeukocyte Common AntigensFemaleCD8Zentralblatt für Gynäkologie
researchProduct

Expression analysis and functional activity of interleukin-7 splice variants.

2008

Alternative splicing results in multiple protein isoforms derived from a single gene. The magnitude of this process ranges from a complete loss of function to gain of new function. We examined, as a paradigm, alternative splicing of the non-redundant human cytokine, interleukin-7 (IL-7). We show that extensive IL-7 splicing in human tissues of different histology, including MTB+ granuloma lesions, transformed tissue and tumor cell lines. IL-7 splice variants were expressed as recombinant proteins. A differentially spliced IL-7 isoform, lacking exon 5, leads to STAT-5 phosphorylation in CD4+ and CD8+ T cells, promotes thymocyte maturation and T-cell survival. Human tumor lesions show aberran…

Gene isoformCD4-Positive T-LymphocytesCell SurvivalImmunologyBiologyCD8-Positive T-LymphocytesExonCell Line TumorGeneticsSTAT5 Transcription FactorHumansProtein IsoformssplicePhosphorylationGenetics (clinical)GranulomaInterleukin-7Alternative splicingInterleukinExonsMolecular biologyRecombinant ProteinsCell biologyThymocyteAlternative SplicingOrgan SpecificityRNA splicingCD8Genes and immunity
researchProduct