Dual-Polarization Interferometry: A Novel Technique To Light up the Nanomolecular World
[EN] The challenging lecture given in 1959 by physicist and Nobel Prize awarded R. P. Feynman: “There's plenty of room at the bottom” is considered to be the starting point for nanotechnology. With this peculiar title, Feynman encouraged researchers to explore beyond the atomic level and predicted exciting new phenomena that might revolutionize science and technology. Among these pioneering researchers are Eric Betzig, Stefan W. Hell and William E. Moerner, who have been awarded with the Nobel Prize in Chemistry 2014 for developing the super-resolved fluorescence microscopy. However, it is important to remark that the exploration of this amazing nanomolecular world began in the early 1980s …
Modeling of the role of conformational dynamics in kinetics of the antigen-antibody interaction in heterogeneous phase.
[EN] A novel approach that may potentially be used to study biomolecular interactions including the simultaneous determination of structural and kinetic binding parameters is described in this Article for the first time. It allows a rigid distinction between the possible reaction mechanisms of biomolecular recognition, induced fit and conformational selection. The relative importance of the two pathways is determined not by comparing rate constants but the structural aspects of the interaction instead. So the exact location of antigen molecules with respect to the capture antibody is depicted experimentally, avoiding the use of X-ray crystallography. The proposed pattern is applied to study…