0000000000605952

AUTHOR

Abdou Elsharawy

showing 2 related works from this author

Mutational Characterization of the Bile Acid Receptor TGR5 in Primary Sclerosing Cholangitis

2010

Background: TGR5, the G protein-coupled bile acid receptor 1 (GPBAR1), has been linked to inflammatory pathways as well as bile homeostasis, and could therefore be involved in primary sclerosing cholangitis (PSC) a chronic inflammatory bile duct disease. We aimed to extensively investigate TGR5 sequence variation in PSC, as well as functionally characterize detected variants.Methodology/Principal Findings: Complete resequencing of TGR5 was performed in 267 PSC patients and 274 healthy controls. Six nonsynonymous mutations were identified in addition to 16 other novel single-nucleotide polymorphisms. To investigate the impact from the nonsynonymous variants on TGR5, we created a receptor mod…

Nonsynonymous substitutionMaleModels MolecularCandidate geneLinkage disequilibriumProtein ConformationDNA Mutational Analysislcsh:MedicineGenome-wide association studySUSCEPTIBILITYMULTIPLE SEQUENCE ALIGNMENTSReceptors G-Protein-CoupledMice0302 clinical medicineChildlcsh:ScienceGenetics and Genomics/Genetics of DiseaseGENE-EXPRESSIONGenetics0303 health sciencesMultidisciplinaryGastroenterology and Hepatology/Biliary TractCROHN-DISEASEMiddle AgedG protein-coupled bile acid receptor3. Good healthGenetics and Genomics/Gene FunctionULCERATIVE-COLITISChromosomes Human Pair 2WEB SERVER030211 gastroenterology & hepatologyFemaleResearch ArticleAdultAdolescentCholangitis SclerosingSingle-nucleotide polymorphismLocus (genetics)BiologyGenetics and Genomics/Complex TraitsPrimary sclerosing cholangitis03 medical and health sciencesYoung AdultDogsPROTEIN-COUPLED RECEPTORSLIVER-DISEASEmedicineAnimalsHumansAmino Acid SequenceBOWEL-DISEASE030304 developmental biologyAgedGastroenterology and Hepatology/Inflammatory Bowel DiseaseCYSTIC-FIBROSISlcsh:Rmedicine.diseaseGene Expression RegulationMutationCancer researchCattleColitis Ulcerativelcsh:Q
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EV-TRACK: transparent reporting and centralizing knowledge in extracellular vesicle research

2017

We argue that the field of extracellular vesicle (EV) biology needs more transparent reporting to facilitate interpretation and replication of experiments. To achieve this, we describe EV-TRACK, a crowdsourcing knowledgebase (http://evtrack.org) that centralizes EV biology and methodology with the goal of stimulating authors, reviewers, editors and funders to put experimental guidelines into practice.

0301 basic medicineminimum informationblood-plasmaphysiology [Extracellular Vesicles]Biomedical ResearchInternationalityComputer sciencephenotype[SDV]Life Sciences [q-bio]MedizinexosomesCrowdsourcingBioinformaticsBiochemistry03 medical and health sciencesExtracellular VesiclesultracentrifugationBiological propertycancerddc:610resolution flow-cytometryMolecular Biologysubpopulationsbusiness.industrybiological-propertiesCell BiologyExtracellular vesicleData scienceDatabases BibliographicReplication (computing)030104 developmental biologycellsbusinessBiotechnology
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