Study of oral microbiota and diet as casual factors of Black Stains in the pediatric patient

The aim of the following study is to identify and quantify microorganisms in the saliva of black stain patients using species-specific probes for Actinomyces israelii, Actinomyces naeslundii, Streptococcus mutans and Lactobacillus spp, as well as a universal probe for bacterial 16S rDNA, to obtain the figure of the total bacterial counts (for test and control group). This study represents a first phase of the research line on extrinsic tooth pigmentation on developmental age patients. Materials and methods. The selected sample is composed of 20 subjects aged between 2 and 15 years and in good general and oral health among the patients included in a dental examination trial at the Department…