0000000000618737

AUTHOR

Josh E. Pérez-ortin

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Sliding-end-labelling

1986

Abstract A method, termed ‘sliding-end-labelling’, has been devised to avoid a frequent artifact in nucleosome positioning by indirect end labelling, namely the appearing of DNA fragments originated by two nuclease cuts, one of them lying within the region covered by the probe. The method is applied to the nucleosome positioning in the yeast SUC2 gene for invertase.

Electrophoresis Agar GelNucleasebiologyBiophysicsNucleic Acid HybridizationDNA Restriction EnzymesSaccharomyces cerevisiaeCell BiologyBiochemistryNucleosomesChromatin Nucleosome positioning Indirect end labelling SUC2 gene (Saccharomyces cerevisiae)BiochemistryStructural BiologyLabellingGeneticsbiology.proteinMicrococcal NucleaseNucleosomeDNA FungalBiological systemMolecular BiologyFEBS Letters
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