NUOVE FRONTIERE DIAGNOSTICHE NELLA TUBERCOLOSI BOVINA. RIVISTA DI PARASSITOLOGIA, vol. VOL. XIII; p. 26-27, ISSN: 0035-6387

Detection of Mycobacterium tuberculosis complex in cattle by PCR on milk, lymphnode and nasal swabs.

The PCR technique was applied to the diagnosis of tuberculosis in live cattle, and both skin-test-negative and skin-test-positive animals were studied. DNA was taken from various sources including specimens of lymph node aspirates, milk, and nasal swabs. After slaughter and visual inspection, tissues such as lymph nodes, lungs, and udders from tuberculin reactors were tested by the same technique. Specific oligonucleotide primers internal to the IS6110 insertion element were used to amplify a 580-bp fragment. A 182-bp fragment was obtained by designating a nested PCR from the first amplification product. This fragment was cloned and sequenced, and after being labeled it was employed in dot …

Purification of a fetal bovine serum factor that inhibits DNA synthesis in chick embryo retinas