0000000000623786
AUTHOR
F. Nuez
Characterization and classification of different genotypes in a population of Cucumis melo based on their ability to regenerate shoots from leaf explants
Variability of the in vitro organogenic response from leaf explants of a seed population of Cueumis melo L. ev. Cantaloupe Charentais was analyzed to detect genotypic variability among individual seeds for shoot regeneration. Variation in shoot regeneration frequency among plants from different seeds was compared to that found among clonal replicas of each original plant. Clonal propagation was performed using in vitro culture of apical and axillary buds of axenic plants to avoid somaclonal variation. Results demonstrate the existence of highly significant differences among plants from the seed population. Regeneration frequency of leaf explants derived from clonally propagated sets of a si…
The inheritance of organogenic response in melon
Previous studies have demonstrated variation in organogenic competence among plants within a population ofCucumis melo. In order to determine if leaf explant response is under genetic control, we investigated the distribution of the shoot regeneration frequency in F1 and F2 generations from parents representing extreme values forin vitro organogenic response. Results suggest a genetic model with two genes, partial dominance, independent segregation and similar effects for both genes.
Correlated response of in vitro regeneration capacity from different source of explants inCucumis melo.
The variation among and within different populations of the regeneration ability from leaf, cotyledon and hypocotyl explants has been studied. A control population and two lines selected by their regeneration capacity from leaf explants were used. Significant differences among the plants of the control population,for the organogenic response, were detected. The regeneration capacity varies depending on the type of explant. Selection in order to improve the regeneration frequency from leaf explants also raises the organogenic response in the other explant types. This result suggests the presence of a partial common genetic system controlling the regeneration frequency of the diverse types of…