0000000000640400
AUTHOR
Monique Charpentier
Characterization of aged champagne wine aroma by GC-O and descriptive profile analyses
Le but du travail est de caracteriser l'evolution de l'arome du champagne au cours du vieillissement et de comparer sous ce rapport le vieillissement accelere a 40 °C au vieillissement normal. Les methodes mises en oeuvre a cet effet sont l'analyse sensorielle, et les techniques GC-O et GC-MS. La representativite des extraits a ete etudiee au prealable par tests triangulaires et tests de similarite. Les profils descriptifs ont ete etablis par un panel d'experts sur des echantillons jeunes et vieillis. L'intensite des notes florale et fruitee diminue au cours du vieillissement, celles des notes boisee et d'evolution augmentent. On observe cependant des differences sensorielles entre les prod…
Purification method for the isolation of monophosphate nucleotides from Champagne wine and their identification by mass spectrometry
Monophosphate nucleotides are difficult to identify in Champagne wine because they are present in small concentrations in a complex mixture. A method for the isolation, separation and identification of reference compounds, which achieved on average 79% recovery (except for cytidine derivatives), was developed and applied to wine. Some monophosphate nucleotides were then isolated from a Champagne wine aged on lees for 8 years, by ultrafiltration followed by a semi-preparative HPLC step using a strong anion-exchange column. The fraction obtained was subjected to HPLC in a reversed-phase column to remove the salt previously introduced, before identification of compounds by HPLC coupled to a ma…