IMPLANTATION OF CARDIAC STEM CELL-LOADED POLY-LACTIC ACID AND FIBRINOIN SCAFFOLDS INTO NUDE MICE TO EVALUATE POTENTIAL FOR CARDIAC MUSCLE TISSUE ENGINEERING

Silk fibroin scaffolds enhance cell commitment of adult rat cardiac progenitor cells.

The use of three-dimensional (3D) cultures may induce cardiac progenitor cells to synthesize their own extracellular matrix (ECM) and sarcomeric proteins to initiate cardiac differentiation. 3D cultures grown on synthetic scaffolds may favour the implantation and survival of stem cells for cell therapy when pharmacological therapies are not efficient in curing cardiovascular diseases and when organ transplantation remains the only treatment able to rescue the patient’s life. Silk fibroin-based scaffolds may be used to increase cell affinity to biomaterials and may be chemically modified to improve cell adhesion. In the present study, porous, partially orientated and electrospun nanometric n…

OBTAINING MESENCHYMAL STEM CELLS FROM ADIPOSE TISSUE OR MURIN ORIGIN: EXPERIMENTAL STUDY.

The aim of this study was to isolate and characterize rat adipose Derived Mesenchymal Stem Cells (AD-MSCs) in order to evaluate their proliferative potential and their ability to different cell types. AD-MSCs and Derived Mesenchymal Stem Cells (BM-MSCs) have the same characteristic in terms of plasticity. The advantage of adipose tissue is that it is an easier accessible source and it offers a large amount of MSCs by less invasive surgical tecniques. MSCs were obtained from subcutaneous adipose tissue of Wistar rats. first of all microbiological controls were made to exclude the presence of bacteria of fungi in then tissue. Adipose tissue was mechanically and enzimatically fragmented and st…

PRODUCTION OF CANINE MESENCHYMAL STEM CELLS FROM ADIPOSE TISSUE AND APPLICATION IN DOGS WITH CHRONIC OSTEOARTHRITIS OF THE HUMERORADIAL JOINTS

Preliminary study on the production of canine mesenchymal stem cells from adipose tissue and possibile applications in dogs with orthopaedic lesions.

The role of biomaterials in the direction of cardiac stem cells properties, differentiation and extracellular matrix interactions in cardiac tissue engineering

Introduction: One of the main problems in the rapid translation of preclinical cell‐based therapy to restore damaged myocardium is to find the best delivery route and the best time of cell injection into the myocardium. Intramyocardial injection of stem cells is by far the mostused delivery technique in preclinical studies. Three‐dimensional scaffolds may be used to deliver a limited number of stem cells in their undifferentiated state, but many biomaterials may cause a foreign body reaction on their own. We have recently demonstrated that c‐Kit positive cardiac progenitor cells are able to organize themselves into a tissue‐like cell mass in collagen I three‐dimensional cultures within 72h …