Author: Olivier Scruel

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AUTHOR

Olivier Scruel

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F-2-isoprostanes: review of analytical methods

2006

International audience; F2-isoprostanes (F2-isoPs) represent a new family of biomarkers for oxidative stress generated by free radical attack of membrane-bounded arachidonic acid. Esterified F2-isoPs can be found in tissue or plasma lipids whereas the free form F2-isoPs, hydrolyzed by phospholipase, is mainly present in body fluids. The extent of systematic damage due to oxidative stress within the body can be assessed by the determination of plasma or urine F2-isoPs. The determination of F2-isoPs in clinical practice is not often used due to the complexity to extract the compounds from their biologic matrixes before the analysis step. In most of published protocols, extraction procedure is…

MASS SPECTROMETRYIsoprostaneBiophysicsPharmaceutical ScienceMass spectrometry01 natural sciencesBiochemistryIMMUNOASSAYchemistry.chemical_compound[ CHIM.ORGA ] Chemical Sciences/Organic chemistryPlasma lipidsQUANTITATIVE ANALYSISNEUROPROSTANESample preparationComputingMilieux_MISCELLANEOUSChromatography010405 organic chemistryChemistry[CHIM.ORGA]Chemical Sciences/Organic chemistryL IPID PEROXIDATION010401 analytical chemistryExtraction (chemistry)[SDV.SP]Life Sciences [q-bio]/Pharmaceutical sciences[CHIM.ORGA] Chemical Sciences/Organic chemistry3. Good health0104 chemical sciencesClinical Practice[SDV.SP] Life Sciences [q-bio]/Pharmaceutical sciencesF2-IsoprostanesMolecular MedicineFree formISOPROSTANE

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PEPhysical Sciences and Engineering
- PE2Fundamental Constituents of Matter
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LSLife Sciences
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SHSocial Sciences and Humanities
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