Genomic characterization of a novel group A lamb rotavirus isolated in Zaragoza, Spain.
An ovine rotavirus (OVR) strain, 762, was isolated from a 30-day-old lamb affected with severe gastroenteritis, in Zaragoza, Spain, and the VP4, VP7, VP6, NSP4, and NSP5/NSP6 genes were subsequently characterized molecularly. Strain OVR762 was classified as a P[14] rotavirus, as the VP4 and VP8* trypsin-cleavage product of the VP4 protein revealed the highest amino acid (aa) identity (94% and 97%, respectively) with that of the P11[14] human rotavirus (HRV) strain PA169, isolated in Italy. Analysis of the VP7 gene product revealed that OVR762 possessed G8 serotype specificity, a type common in ruminants, with the highest degree of aa identity (95–98%) shared with serotype G8 HRV, bovine rot…
User acceptance of automated public transport
Abstract Autonomous driving is receiving increasing attention in the automotive industry as well as in public transport. However, it is still unclear whether users are willing to use automated public transportation at all. In order to answer this and other questions, the transport company of the city of Mainz, Germany, tested the autonomous minibus EMMA (Elektro-Mobilitat Mainz Autonom) on a 600-meter-long test track in public space. The study presented here was conducted with the aim of exploring crucial determinants for the use of an autonomous minibus. On the basis of established acceptance models, a questionnaire was developed, which was completed in a field survey by a total of 942 par…