0000000000699283
AUTHOR
L Schena
Identification of Phytophthora species infecting citrus in Vietnam
In a survey aimed at identifying Phytophthora species infecting fruit crops in Viet Nam, P. citrophthora and P. nicotianae were the species most commonly recovered from soil and trees in citrus plantations. P. nicotianae was prevalent in citrus groves in the Ben Tre and Tien Giang provinces (southern Viet Nam). Interestingly, both A1 and A2 mating types of the latter species were found, while in other citrus-growing areas of the world, such as the Mediterranean region, South Africa, Australia and the Americas, A1 is the only mating type occurring in citrus groves, suggesting southern-east Asia could be a centre of origin of this Phytophthora species. Mixed infections of three Phytophthora s…
Whither the species? Phytophthora taxa, MOTUs and barcodes in the world of metagenomics
The study of ecosystem diversity is being transformed by high throughput sequencing technology that allows an unparalleled depth of sampling of DNA barcode sequences. The opportunities are great at this fascinating interface of pathology, ecology, taxonomy and molecular biology but there are pitfalls. It is important to consider the potential for bias at all steps in the process from sampling through to data analysis. Increasingly Phytophthora diversity is being examined by metabarcoding of the PCR amplified rDNA ITS regions from soil, water or plant sample DNA. We have applied baiting and isolation, cloning and Sanger sequencing and Illumina MiSeq analysis to a time-series of filtered wate…