0000000000701367

AUTHOR

Alexandra K. Kiemer

0000-0002-7224-9900

showing 1 related works from this author

BCL-2 UPREGULATION AFTER 3-NITROPROPIONIC ACID PRECONDITIONING IN WARM RAT LIVER ISCHEMIA

2008

We aimed to determine whether 3-nitropropionic acid (3-NPA) preconditioning protects rat livers against warm ischemia/reperfusion injury. We hypothesized that 3-NPA mediates its protective effects by Bcl-2 upregulation. Brown-Norway rats (200 g) were injected with 3-NPA (10 mg/kg intraperitoneally) 24 h before 90 min of selective warm in situ ischemia. In additional experiments, 30-day survival was studied after 90 min of warm liver ischemia and resection of nonischemic liver tissue. We demonstrate increased mRNA and protein levels of Bcl-2 by real-time polymerase chain reaction, immunohistochemistry, and Western blot analysis in 3-NPA-pretreated rats. All treated animals survived, whereas …

Malemedicine.medical_specialtyBlotting WesternIschemiaCritical Care and Intensive Care MedicineLipid peroxidationchemistry.chemical_compoundstomatognathic systemWestern blotDownregulation and upregulationInternal medicinemedicineAnimalsWarm IschemiaIschemic PreconditioningCaspasechemistry.chemical_classificationReactive oxygen speciesmedicine.diagnostic_testbiologyCaspase 3Reverse Transcriptase Polymerase Chain ReactionNitro Compoundsmedicine.diseaseImmunohistochemistryCaspase 9RatsEndocrinologyLiverProto-Oncogene Proteins c-bcl-2chemistryApoptosisReperfusion InjuryEmergency Medicinebiology.proteinPropionatesReperfusion injuryShock
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