0000000000707464
AUTHOR
Jordi Ventura
Additional file 2 of Probing the operability regime of an engineered ribocomputing unit in terms of dynamic range maintenance with extracellular changes and time
Additional file 2: Fig. S2. Evolutionary stability of a control system (E. coli transformed with plasmid pRHA27). Continuous fluorescence monitoring of four biological replicates (A-D) over one week.
Additional file 1 of Probing the operability regime of an engineered ribocomputing unit in terms of dynamic range maintenance with extracellular changes and time
Additional file 1: Fig. S1. Environmental robustness assessment of a control system (E. coli transformed with plasmid pRHA27; this system just expresses sfGFP under the control of the lac promoter). Fluorescence monitoring (normalized green fluorescence) when the culture medium changes (pH and temperature variation). Error bars correspond to standard errors over four biological replicates.
Additional file 3 of Probing the operability regime of an engineered ribocomputing unit in terms of dynamic range maintenance with extracellular changes and time
Additional file 3: Fig. S3. Schematics of the plasmids pRHA40, pRHA12, and pRHA1240.
Probing the operability regime of an engineered ribocomputing unit in terms of dynamic range maintenance with extracellular changes and time
Synthetic biology aims at engineering gene regulatory circuits to end with cells (re)programmed on purpose to implement novel functions or discover natural behaviors. However, one overlooked question is whether the resulting circuits perform as intended in variety of environments or with time. Here, we considered a recently engineered genetic system that allows programming the cell to work as a minimal computer (arithmetic logic unit) in order to analyze its operability regime. This system involves transcriptional and post-transcriptional regulations. In particular, we studied the analog behavior of the system, the effect of physicochemical changes in the environment, the impact on cell gro…
Additional file 4 of Probing the operability regime of an engineered ribocomputing unit in terms of dynamic range maintenance with extracellular changes and time
Additional file 4: Dataset S1. Experimental and theoretical data of the half adder behavior. The Excel file contains an implementation of the mathematical model.