0000000000712827
AUTHOR
Maurizio Micheli
Effetti di diverse concentrazioni di NaCl applicate su Morus nigra L. attraverso la tecnologia dell’incapsulamento
In questo studio è stata valutata l’influenza di differenti condizioni di stress salino sul genotipo “Fontanarossa nera” (Morus nigra L.), applicando la tecnologia del seme sintetico. Le concentrazioni di NaCl pari a 0, 50, 100, 150 e 200 mM, sono state aggiunte alle tre soluzioni (incapsulante, complessante e di lavaggio) costituenti l’endosperma artificiale, utilizzato per rivestire microtalee uninodali di 4 mm di lunghezza. Dopo 45 giorni dalla semina su substrato privo di ormoni, sono state registrate le percentuali di vitalità, ripresa e conversione ed i parametri morfologici (numero e lunghezza di germogli e di radici, callo). Sono state effettuate inoltre, analisi per la stima di ind…
STUDY ON CONVERSION OF ENCAPSULATED VITRO-DERIVED PROPAGULES OF 'C35' CITRANGE [C. SINENSIS (L.) OSB. × P. TRIFOLIATE (L.) RAF.]
Fight against Citrus tristeza virus (CTV) implies the use of CTV-resistant rootstocks, such as citranges [C. Sinensis (L.) Osb. × P. Trifoliata (L.) Raf.]. The 'C35' represents a promising tolerant to CTV genotype. The employment of innovative in vitro techniques, such as encapsulation, could open new perspectives for this genotype propagation, particularly in the nursery activity. Preliminary experiments on calcium-alginate encapsulation of microcuttings (unipolar propagules) have already been performed to evaluate the use of tissue culture for the propagation and the preservation of different citrange rootstocks: Carrizo and Troyer. In the present study, the influence of different types a…
Research on conversion of Carrizo citrange [C. sinensis (L.) Osb. × P. trifoliata (L.) Raf.] synthetic seeds.
Organogenesis and encapsulation of in vitro-derived propagules of Carrizo citrange [Citrus sinensis (L.) Osb. × Poncirius trifoliata (L.) Raf]
Due to widespread polyembryony, Citrus rootstocks are usually propagated by open-pollinated seed germination, although micropropagation offers many advantages. Encapsulation technology has recently attracted the interest of researchers in the field of plant propagation because it combines the advantages of zygotic or gamic seeds with those of micropropagation. In this study, we examined the encapsulation of Carrizo citrange uninodal microcuttings (3–4 mm long) and evaluated the influence of the calcium alginate coating, a short time storage at cold temperature, and different sowing substrates on the viability and regrowth of the explants. A secondary aim was to develop an efficient protocol…
Perspectives of the encapsulation technology in the nursery activity of Citrus
The genetic and phytosanitary certification has to guarantee the quality of the propagation material also in Citrus. Traditional methods can be assisted by the techniques of in vitro culture, which are fundamental for the mass production of high quality plants. The encapsulation technology represents an innovative tool to combine the advantages of micropropagation with handling ease, storability, reduced size of the propagules, mechanization potentiality, transportability of the gamic seeds. In this work somatic embryos obtained from in vitro anther culture of the cultivar Mandarino Tardivo di Ciaculli (Citrus reticulata Blanco) were encapsulated in a sodium alginate matrix and synthetic se…