0000000000717373
AUTHOR
V Arena
Tecnica del sovracarotaggio per pilastri compressi in c.a.
The paper shows the results of an experimental investigation on the behaviour of compressed concrete columns subjected to the overcoring technique. Extraction of cylindrical concrete cores in perpendicular direction to the column surface, was achieved by rotary drilling using a diamond tipped hollow barrel. Core is gradually isolated from the stress field in the surrounding concrete and at every advancing step the re-equilibrium deformation response is measured by means of four strain-gauges, placed on its external surface and near the hole. Finally the original stress state is computed in the elastic phase on the basis of recorded strains, knowing the elastic properties of concrete and by …
In vitro models of blood-brain barrier set by co-culturing primary pericytes, astrocytes and brain capillary endothelial cells
The blood-brain barrier (BBB) is formed by brain capillary endothelial cells (BCECs) under the effects of the brain microenvironment. BCECs are sealed together by tight junctions (TJs) [2]. We previously found that occludin, one of the main TJ components, is correctly localized at the cell periphery only if BCECs are co-cultured with neurons/astrocytes [1-2]. Moreover, pericytes induce PLA2 protein expression through activation of PKCalpha and the MAPK/ERK cascade in immortalized GP8.3 endothelial cells [3]. In the present work, we analyzed the effects of pericytes and/or astrocytes on survival and differentiation of primary endothelial cells, in the presence of a serum-free medium. To test…