Determination of the adiabatic compressibility of bovine serum albumen in concentrated solution by a new ultrasonic method

2000

Abstract New data are presented for the adiabatic compressibility of bovine serum albumen (BSA) over the temperature range 2–85°C and at pH 5, 6, 7 and 8. The relative impact of intrinsic and hydration contributions to the observed compressibility for BSA is estimated and shown to agree very well with general values for globular protein and with previous results for BSA. The compressibility manifests a maximum value in the regions where BSA is most stable, exhibiting a parabolic dependence on temperature with lowest values where it is least stable. These data are obtained with a novel technique that employs a commercial, low cost and automated pulse echo apparatus for the measurement of the…