0000000000733361

AUTHOR

Jean Menotti

showing 2 related works from this author

Evaluation of five automated and one manual method for Toxoplasma and human DNA extraction from artificially spiked amniotic fluid.

2018

International audience; Objectives - Molecular detection of Toxoplasma gondii plays a crucial role in the prenatal and neonatal diagnosis of congenital toxoplasmosis (CT). Sensitivity of this diagnosis is partly related to the efficiency of parasite DNA extraction and amplification. DNA extraction methods with automated platforms have been developed. Therefore, it is essential to evaluate them in combination with adequate PCR amplification assays.Methods - In this multisite study, we investigated the suitability of two recent automated procedures for the isolation of Toxoplasma DNA from amniotic fluid (AF) (Magtration system 12GC, PSS and Freedom EVO VacS, Tecan), compared with three other …

0301 basic medicineMicrobiology (medical)[ SDV.MP.PAR ] Life Sciences [q-bio]/Microbiology and Parasitology/ParasitologyAmniotic fluid030106 microbiologyToxoplasma gondiiPolymerase Chain ReactionSensitivity and SpecificityToxoplasmosis Congenitallaw.invention03 medical and health scienceschemistry.chemical_compound0302 clinical medicinelawparasitic diseasesDiagnosisTaqManHumans[SDV.MP.PAR]Life Sciences [q-bio]/Microbiology and Parasitology/Parasitology030212 general & internal medicineDNA extractionPolymerase chain reactionChromatographyCongenital toxoplasmosisbiologyExtraction (chemistry)Toxoplasma gondiiNucleic Acid Hybridization[ SDV.SPEE ] Life Sciences [q-bio]/Santé publique et épidémiologieGeneral Medicinerep529DNADNA Protozoanbiology.organism_classificationAmniotic FluidDNA extractionCongenital toxoplasmosisrap5293. Good healthInfectious DiseasesPCRchemistry[SDV.SPEE]Life Sciences [q-bio]/Santé publique et épidémiologieBiological AssayReagent Kits DiagnosticToxoplasmaDNAClinical microbiology and infection : the official publication of the European Society of Clinical Microbiology and Infectious Diseases
researchProduct

Molecular Diagnosis of Toxoplasmosis in Immunocompromised Patients: a 3-Year Multicenter Retrospective Study

2015

ABSTRACT Toxoplasmosis is a life-threatening infection in immunocompromised patients (ICPs). The definitive diagnosis relies on parasite DNA detection, but little is known about the incidence and burden of disease in HIV-negative patients. A 3-year retrospective study was conducted in 15 reference laboratories from the network of the French National Reference Center for Toxoplasmosis, in order to record the frequency of Toxoplasma gondii DNA detection in ICPs and to review the molecular methods used for diagnosis and the prevention measures implemented in transplant patients. During the study period, of 31,640 PCRs performed on samples from ICPs, 610 were positive (323 patients). Blood ( n …

Microbiology (medical)Microbiological Techniquesmedicine.medical_specialtyMESH: Molecular Diagnostic TechniquesAsymptomaticPolymerase Chain Reactionlaw.inventionImmunocompromised Host[SDV.MHEP.MI]Life Sciences [q-bio]/Human health and pathology/Infectious diseaseslawInternal medicinemedicineMESH: Immunocompromised HostPrevalenceHumansComputingMilieux_MISCELLANEOUSPolymerase chain reactionImmunodeficiencySurvival analysisMESH: PrevalenceRetrospective Studies[SDV.EE.SANT]Life Sciences [q-bio]/Ecology environment/HealthMESH: Humansbusiness.industryIncidence (epidemiology)MESH: ToxoplasmaMESH: Microbiological TechniquesRetrospective cohort studyMESH: Polymerase Chain ReactionMESH: Retrospective Studiesmedicine.diseaseSurvival AnalysisToxoplasmosis3. Good healthSurgeryMESH: France[SDV.MP]Life Sciences [q-bio]/Microbiology and ParasitologyMolecular Diagnostic TechniquesMESH: Survival AnalysisChemoprophylaxisMESH: ToxoplasmosisParasitologyFrancemedicine.symptombusinessToxoplasmaToxoplasmosis
researchProduct