0000000000740065
AUTHOR
Borras Consuelo
Alpha-(13′-hydroxy)-6-hydroxychroman, the main product of alpha-tocopherol metabolism in human hepatocytes, regulates CYP4F2 and PPAR-γ expression
The enzymatic metabolism of vitamin E in liver cells generates long chain metabolites (LCMs) with proposed regulatory activity on inflammatory and atherogenic genes. In this study the LCM formation kinetics was characterized in HepG2 and HepaRG human hepatic cells, supplemented with RRR-α-tocopherol (α-TOH). α-13’OH was the main product of α-TOH metabolism, while α-13’COOH metabolite and the short chain metabolite α-CEHC, were detected only in traces, thus demonstrating the poor efficiency of vitamin E catabolism in these cells. However, this metabolism was significantly simulated when the hepatic cells were challenged with (lipo)toxic agents, such as ethanol or palmitate. Under such condit…
Centenarians, but not octogenarians, up-regulate the expression of microRNAs
Centenarians exhibit extreme longevity and a remarkable compression of morbidity. They have a unique capacity to maintain homeostatic mechanisms. Since small non-coding RNAs (including microRNAs) are implicated in the regulation of gene expression, we hypothesised that longevity of centenarians may reflect alterations in small non-coding RNA expression. We report the first comparison of microRNAs expression profiles in mononuclear cells from centenarians, octogenarians and young individuals resident near Valencia, Spain. Principal Component Analysis of the expression of 15,644 mature microRNAs and, 2,334 snoRNAs and scaRNAs in centenarians revealed a significant overlap with profiles in you…