Expression and regulation of PAX-9 and MSX-1 in embryonic and post-natal dental stem cells.

Introduction: Msx1and Pax9, are genes expressed in mesenchymal cells during the odontogenesis that are required for tooth formation. Our investigation aimed to study the expression of these genes in two sources of dental mesenchymal cells. Stem Cells from Human Exfoliated Deciduous teeth (SHED) and Human Embryonic Dental Mesenchymal Cells (HTM) were used. Regulation of gene expression in these cells by FGF, Wnt, BMP4 and Shh signalling pathways together with the effects of tooth matrix proteins (EMD) was investigated. Materials and Methods: Cells were grown in 6 well plates in 3 ml MSCGM medium (Lonza) containing Glutamax, 10% FBS and gentamicin Sulfate. Cells were grown to confluence and w…