Impact of Titanium Dioxide Nanoparticle Dispersion State and Dispersion Method on Their Toxicity Towards A549 Lung Cells and Escherichia coli Bacteria

International audience; Titanium-dioxide nanoparticle (TiO2-NPs) dispersion for toxicological studies is classically achieved by high power sonication. The aim of this study was to compare the dispersion state resulting from different dispersion techniques, and to correlate dispersion state and dispersion method to in vitro toxicity in a final view to contribute to the very active field of establishing protocols for reliable NP toxicity testing. To achieve this objective, several dispersion methods were applied to Evonik P25 TiO2-NPs. The dispersion state, as well as the stability of these TiO2-NP suspensions were evaluated by photon correlation spectroscopy (PCS) and turbidimetry. A549 hum…