0000000000752620
AUTHOR
Strategic Infection Biology
Single-cell analysis of population context advances RNAi screening at multiple levels
Isogenic cells in culture show strong variability, which arises from dynamic adaptations to the microenvironment of individual cells. Here we study the influence of the cell population context, which determines a single cell's microenvironment, in image‐based RNAi screens. We developed a comprehensive computational approach that employs Bayesian and multivariate methods at the single‐cell level. We applied these methods to 45 RNA interference screens of various sizes, including 7 druggable genome and 2 genome‐wide screens, analysing 17 different mammalian virus infections and four related cell physiological processes. Analysing cell‐based screens at this depth reveals widespread RNAi‐induce…
Vesiclepedia:A Compendium for Extracellular Vesicles with Continuous Community Annotation
Vesiclepedia is a community-annotated compendium of molecular data on extracellular vesicles.