Author: Julien Bouckenheimer

0000000000755097

AUTHOR

Julien Bouckenheimer

showing 1 related works from this author

Differential long non-coding RNA expression profiles in human oocytes and cumulus cells

2018

AbstractProgress in assisted reproductive technologies strongly relies on understanding the regulation of the dialogue between oocyte and cumulus cells (CCs). Little is known about the role of long non-coding RNAs (lncRNAs) in the human cumulus-oocyte complex (COC). To this aim, publicly available RNA-sequencing data were analyzed to identify lncRNAs that were abundant in metaphase II (MII) oocytes (BCAR4, C3orf56, TUNAR, OOEP-AS1, CASC18, and LINC01118) and CCs (NEAT1, MALAT1, ANXA2P2, MEG3, IL6STP1, and VIM-AS1). These data were validated by RT-qPCR analysis using independent oocytes and CC samples. The functions of the identified lncRNAs were then predicted by constructing lncRNA-mRNA co…

0301 basic medicine[SDV]Life Sciences [q-bio]lcsh:MedicineReproductive technologyBiologyReal-Time Polymerase Chain ReactionArticleChromatin remodeling03 medical and health sciencesmedicineHumanslcsh:ScienceGeneMetaphaseMEG3MALAT1Cumulus CellsMultidisciplinaryReverse Transcriptase Polymerase Chain ReactionGene Expression Profilinglcsh:RComputational BiologyOocyteLong non-coding RNACell biology[SDV] Life Sciences [q-bio]Gene expression profiling030104 developmental biologymedicine.anatomical_structureOocytesRNA Long Noncodinglcsh:Q

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PEPhysical Sciences and Engineering
- PE2Fundamental Constituents of Matter
  - PE2_1Theory of fundamental interactions
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- PE1Mathematics
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LSLife Sciences
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SHSocial Sciences and Humanities
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