0000000000757270

AUTHOR

Rosanna La Rocca

showing 2 related works from this author

Human NK cells selective targeting of colon cancer-initiating cells: a role for natural cytotoxicity receptors and MHC class I molecules

2013

Abstract Tumor cell populations have been recently proposed to be composed of two compartments: tumor-initiating cells characterized by a slow and asymmetrical growth, and the “differentiated” cancer cells with a fast and symmetrical growth. Cancer stem cells or cancer-initiating cells (CICs) play a crucial role in tumor recurrence. The resistance of CICs to drugs and irradiation often allows them to survive traditional therapy. NK cells are potent cytotoxic lymphocytes that can recognize tumor cells. In this study, we have analyzed the NK cell recognition of tumor target cells derived from the two cancer cell compartments of colon adenocarcinoma lesions. Our data demonstrate that freshly p…

Cytotoxicity ImmunologicNKImmunologyGene ExpressionCancer Stem CellMice SCIDBiologyAdenocarcinomaInterleukin 21MiceNK-92Cancer stem cellMice Inbred NODTumor Cells CulturedImmunology and AllergyCytotoxic T cellAnimalsHumansCell LineageSettore MED/04 - Patologia GeneraleLymphokine-activated killer cellMicroscopy ConfocalNatural Cytotoxicity Triggering Receptor 3Natural Cytotoxicity Triggering Receptor 2Janus kinase 3Histocompatibility Antigens Class Inessuna parola chiaveKiller Cells NaturalOrgan SpecificityImmunologyCancer cellColonic NeoplasmsCancer researchInterleukin 12Neoplastic Stem Cellsimmunotherapy
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Lipid Droplets: A New Player in Colorectal Cancer Stem Cells Unveiled by Spectroscopic Imaging

2015

Abstract The cancer stem cell (CSC) model is describing tumors as a hierarchical organized system and CSCs are suggested to be responsible for cancer recurrence after therapy. The identification of specific markers of CSCs is therefore of paramount importance. Here, we show that high levels of lipid droplets (LDs) are a distinctive mark of CSCs in colorectal (CR) cancer. This increased lipid content was clearly revealed by label-free Raman spectroscopy and it directly correlates with well-accepted CR-CSC markers as CD133 and Wnt pathway activity. By xenotransplantation experiments, we have finally demonstrated that CR-CSCs overexpressing LDs retain most tumorigenic potential. A relevant con…

Colorectal cancerXenotransplantationmedicine.medical_treatmentBiologySpectrum Analysis RamanMiceCancer stem cellLipid dropletOrganelleBiomarkers TumormedicineAnimalsHumanslipid droplets colon cancer stem cellsWnt Signaling PathwaySettore MED/04 - Patologia GeneraleWnt signaling pathwayCancerLipid DropletsCell Biologymedicine.diseaseCell biologyNeoplastic Stem CellsMolecular MedicineStem cellColorectal NeoplasmsDevelopmental BiologyStem Cells
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