0000000000792256
AUTHOR
A Notaro
p8 (candidate of metasiasis 1) drives ER-stress/autophagy/apoptosis axis induced by the synthetic cannabinoid WIN in HCC cells.
Background: Today, evidence is emerging for the role of autophagy in the regulation of life and death of tumour cells and its relationship with ER-stress signaling. Our previous results demonstrated that hepatoma HepG2 cells are sensitive to apoptotic effects induced by WIN, a synthetic cannabinoid, which acts through a mechanism involving the reduction in the levels of some survival factors and the activation of pro-apoptotic ones. Since WIN effects were observed after 36−48 hours of treatment, we investigated the possible activation of ER-stress and autophagic process in the first hours of WIN treatment focusing our attention on p8, a factor whose expression is upregulated in response to …
Cannabinoid-associated cell death mechanisms in tumor models (review)
In recent years, cannabinoids (the active components of Cannabis sativa) and their derivatives have received considerable interest due to findings that they can affect the viability and invasiveness of a variety of different cancer cells. Moreover, in addition to their inhibitory effects on tumor growth and migration, angiogenesis and metastasis, the ability of these compounds to induce different pathways of cell death has been highlighted. Here, we review the most recent results generating interest in the field of death mechanisms induced by cannabinoids in cancer cells. In particular, we analyze the pathways triggered by cannabinoids to induce apoptosis or autophagy and investigate the in…
COINVOLGIMENTO DELLO STRESS DEL RETICOLO E DEL PROCESSO AUTOFAGICO NELL’APOPTOSI INDOTTA DAL CANNABINOIDE SINTETICO WIN IN CELLULE DI EPATOMA UMANO IN COLTURA
Studi da noi condotti precedentemente hanno dimostrato la capacità del cannabinoide sintetico WIN di indurre apoptosi in cellule di epatocarcinoma umano HepG2 attraverso un meccanismo, dipendente dal fattore trascrizionale PPARg, che prevede riduzione dei livelli di alcuni fattori di sopravvivenza e attivazione di fattori pro-apoptotici della famiglia Bcl-2 (M. Giuliano et al. Biochimie. 2009). Recentemente è, inoltre, emerso che in cellule di glioma i cannabinoidi possono stimolare l’apoptosi attraverso induzione di stress del reticolo endoplasmatico seguito da autofagia. Scopo L'obiettivo del presente studio è stato quello di valutare il coinvolgimento dell’autofagia nel percorso di morte…