0000000000795515

AUTHOR

Jorge Babul

showing 2 related works from this author

Studying the phosphoryl transfer mechanism of the E. coli phosphofructokinase-2: from X-ray structure to quantum mechanics/molecular mechanics simula…

2019

Phosphofructokinases (Pfks) catalyze the ATP-dependent phosphorylation of fructose-6-phosphate (F6P) and they are regulated in a wide variety of organisms. Although numerous aspects of the kinetics and regulation have been characterized for Pfks, the knowledge about the mechanism of the phosphoryl transfer reaction and the transition state lags behind. In this work, we describe the X-ray crystal structure of the homodimeric Pfk-2 from E. coli, which contains products in one site and reactants in the other, as well as an additional ATP molecule in the inhibitory allosteric site adjacent to the reactants. This complex was previously predicted when studying the kinetic mechanism of ATP inhibit…

Reaction mechanism010405 organic chemistryChemistryMetaphosphateKineticsAllosteric regulationGeneral Chemistry010402 general chemistry01 natural sciencesMolecular mechanics0104 chemical sciencesMolecular dynamicschemistry.chemical_compoundBACTÉRIAS GRAM-NEGATIVASQuantum mechanicsMoleculePhosphofructokinasesChemical Science
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Studying the phosphoryl transfer mechanism of the

2019

Phosphofructokinases catalyze the ATP-dependent phosphorylation of fructose-6-phosphate and they are highly regulated.

inorganic chemicalsenzymes and coenzymes (carbohydrates)Chemistrybacteriamacromolecular substancesenvironment and public healthChemical science
researchProduct