0000000000813889

AUTHOR

Pierre Larraufie

showing 3 related works from this author

Quantitative mass spectrometry for human melanocortin peptides in vitro and in vivo suggests prominent roles for b-MSH and desacetyl a-MSH in energy …

2018

Objective: The lack of pro-opiomelanocortin (POMC)-derived melanocortin peptides results in hypoadrenalism and severe obesity in both humans and rodents that is treatable with synthetic melanocortins. However, there are significant differences in POMC processing between humans and rodents, and little is known about the relative physiological importance of POMC products in the human brain. The aim of this study was to determine which POMC-derived peptides are present in the human brain, to establish their relative concentrations, and to test if their production is dynamically regulated. Methods: We analysed both fresh post-mortem human hypothalamic tissue and hypothalamic neurons derived fro…

Leptinendocrine systemNeuropeptideintegumentary systemdigestive oral and skin physiologyObesitatPOMCPèptidsHuman pluripotent stem cellObesityMSHhormones hormone substitutes and hormone antagonists
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Quantitative mass spectrometry for human melanocortin peptides in vitro and in vivo suggests prominent roles for β-MSH and desacetyl α-MSH in energy …

2018

Objective The lack of pro-opiomelanocortin (POMC)-derived melanocortin peptides results in hypoadrenalism and severe obesity in both humans and rodents that is treatable with synthetic melanocortins. However, there are significant differences in POMC processing between humans and rodents, and little is known about the relative physiological importance of POMC products in the human brain. The aim of this study was to determine which POMC-derived peptides are present in the human brain, to establish their relative concentrations, and to test if their production is dynamically regulated. Methods We analysed both fresh post-mortem human hypothalamic tissue and hypothalamic neurons derived from …

MalePluripotent Stem CellsLeptinlcsh:Internal medicineendocrine systemhPSC human pluripotent stem cellsPro-Opiomelanocortin[SDV.NEU.NB]Life Sciences [q-bio]/Neurons and Cognition [q-bio.NC]/NeurobiologyHypothalamusMass SpectrometryTandem Mass Spectrometry[SDV.BBM.GTP]Life Sciences [q-bio]/Biochemistry Molecular Biology/Genomics [q-bio.GN]beta-MSHHomeostasisHumansHuman pluripotent stem cellObesitylcsh:RC31-1245MSHNeuronsintegumentary systemReceptors MelanocortinLC-MS/MS liquid chromatography tandem mass spectrometryNeuropeptidesdigestive oral and skin physiologyPOMCPVH the paraventricular nucleus of the hypothalamusCTX cerebral cortexMelanocortinsNeuropeptidealpha-MSHOriginal ArticleFemalehormones hormone substitutes and hormone antagonistsChromatography Liquid
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Human POMC processing in vitro and in vivo revealed by quantitative peptidomics

2018

ABSTRACTHuman obesity can result from the aberrant production or processing of proopiomelanocortin (POMC) in hypothalamic neurons, but it is unclear which human POMC-derived peptides are most relevant to body weight regulation. To address this question, we analysed both hypothalamic neurons derived from human pluripotent stem cells (hPSCs) and primary human hypothalamic tissue using quantitative liquid chromatography tandem mass spectroscopy (LC-MS/MS). In both in vitro- and in vivo-derived samples, we found that POMC was processed into β-melanocyte stimulating hormone (β-MSH), whose existence in the human brain has been controversial. β-MSH and desacetyl α-MSH (d-α-MSH) were produced at ro…

0303 health sciencesendocrine systembiologyintegumentary systemChemistryHuman brainIn vitroCell biology03 medical and health sciences0302 clinical medicinemedicine.anatomical_structureProopiomelanocortinAcetylationIn vivomedicinebiology.proteinSecretionInduced pluripotent stem cell030217 neurology & neurosurgeryhormones hormone substitutes and hormone antagonists030304 developmental biologyHormone
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