Microscopy method and apparatus for optical tracking of emitter objects

Microscopy method and apparatus for determining the posi tions of emitter objects in a three - dimensional space that comprises focusing scattered light or fluorescent light emitted by an emitter object, separating the focused beam in a first and a second optical beam, directing the first and the second optical beam through a varifocal lens having an optical axis such that the first optical beam impinges on the lens along the optical axis and the second beam impinges decentralized with respect to the optical axis of the varifocal lens , simultaneously capturing a first image created by the first optical beam and a second image created by the second optical beam , and determining the relativ…