0000000000827966
AUTHOR
Christine Schmitt
Tissue response to five commercially available peritoneal adhesion barriers-A systematic histological evaluation
Separating wounded serosa by physical barriers is the only clinically approved adjunct for postoperative adhesion prevention. Since the optimal adhesion barrier has not been found, it is essential to improve our pathogenic understanding of adhesion formation and to compare the effects of different barrier materials on tissue and cells. Wistar rats underwent standardized peritoneal damage and were treated either with Seprafilm, Adept, Intercoat, Spraygel, SupraSeal or remained untreated as a control. 14 days postoperatively, the lesions were explanted and histomorphologically analyzed using the European ISO score to evaluate material implants. Striking differences between the material groups…
LATE-BREAKING ABSTRACT: Comparative studies on bronchuswall-thickness by histologic and computed tomographic measurements of porcine lungs
Aims: Histologic slides are commonly used as template in the evaluation and development of medical imaging methods.Diseases like Asthma and COPD show characteristic changes in airway morphology and airway measurement by computed tomography is a promising diagnostic approach.However,shrinkage caused by fixation and histological processing is known in lung tissue.In this study,the thickness of bronchus walls in paraffin and frozen sections as well as in CT and MicroCT were compared. Methods: Airway measurements of swine lungs were performed after freezing in ventilated condition in liquid nitrogen by measuring the wall thickness of 7 bronchi via CT and MicroCT as well as in frozen and paraffi…
Tissue expansion of lung bronchi due to tissue processing for histology – A comparative analysis of paraffin versus frozen sections in a pig model
Tissue shrinking due to fixation and processing is well known. However, the degree of shrinking varies significantly with the tissue type as well as the processing method and is not well studied in various tissues. In daily pathological routine workflow, histological specimens from frozen and paraffin sections are performed from the same tissue. In the present study we compared the thickness of bronchus walls obtained from paraffin and frozen sections.Pig lungs were frozen in ventilated condition in liquid nitrogen and 36 bronchi were isolated after dissection. Frozen sections of 5 μm thickness were performed and the remaining tissue was fixed and embedded in paraffin after fixation in 4% f…
Comparison of histological and computed tomographic measurements of pig lung bronchi.
Aim Light microscopy is used as template in the evaluation and further development of medical imaging methods. Tissue shrinkage caused by histological processing is known to influence lung tissue dimensions. In diagnosis of COPD, computed tomography (CT) is widely used for automated airway measurement. The aim of this study was to compare histological and computed tomographic measurements of pig lung bronchi. Methods Airway measurements of pig lungs were performed after freezing under controlled inflation pressure in a liquid nitrogen bath. The wall thickness of seven bronchi was measured via Micro-CT and CT using the integral-based method (IBM) and the full-width-at-half-maximum method (FW…