0000000000860785
AUTHOR
Michael R. Wolf
Endothelialization of a non-woven silk fibroin net for use in tissue engineering: growth and gene regulation of human endothelial cells
Abstract We have previously shown that a biomaterial consisting of a non-woven fibroin net produced from silk (Bombyx mori) cocoons is an excellent scaffolding material for a wide variety of human cells of different tissue types. Endothelialization must take place for a biomaterial to be successful after implantation. Therefore, primary human endothelial cells and the human endothelial cell lines, HPMEC-ST1.6R and ISO-HAS-1, were examined for adherence and growth patterns on the fibroin nets by confocal laser scanning microscopy after vital staining of the cells and by electron microscopy. Endothelial cells adhered and spread along individual fibers of the nets and did not fill the gaps bet…
Growth of human cells on a non-woven silk fibroin net: a potential for use in tissue engineering.
We have examined a novel biomaterial consisting of a non-woven fibroin net produced from silk (Bombyx mori) cocoons for its ability to support the growth of human cells. Various human cells of different tissue and cell types (endothelial, epithelial, fibroblast, glial, keratinocyte, osteoblast) were examined for adherence and growth on the nets by confocal laser microscopy after staining of the cells with calcein-AM and by electron microscopy. All the cells readily adhered and spread over the individual fibers of the nets. Most of the cells were able to grow and survive on the nets for at least 7 weeks and growth not only covered the individual fibers of the net but generally bridged the ga…
Political Discussion in Modern Democracies
1. Introduction: Political Discussion in Modern Democracies from a Comparative Perspective Michael R. Wolf and Ken'ichi Ikeda Part 1: Deliberation and Discussion as the Object of Analysis 2. Dissecting Deliberative Democracy: A Review of Theoretical Concepts and Empirical Findings Andre Bachtiger and Seraina Pedrini 3. The Equality Paradox of Deliberative Democracy: Evidence from a National Deliberative Poll Kasper Moller Hansen 4. What Political Discussion Means and How Do the French and (French-Speaking) Belgians Deal with It? Sophie Duchesne and Florence Haegel 5. Participatory Budgeting, Discussion Networks and Political Information in Two Brazilian Cities Lucio R. Renno and Barry Ames …