0000000000871918

AUTHOR

Agustín Ballesteros

showing 3 related works from this author

Transcriptomic behavior of genes associated with chromosome 21 aneuploidies in early embryo development.

2019

To analyze how chromosome 21 (HSA21) ploidy affects global gene expression of early human blastocysts.Prospective study.University-affiliated in vitro fertilization clinic.A total of 26 high-quality donated embryos from in vitro fertilization (IVF) patients: trisomy 21 (n = 8), monosomy 21 (n = 10), and euploid (n = 8) blastocysts.None.Blastocyst transcriptome changes and its associated functions.Trisomy 21, monosomy 21, and euploid blastocysts were classified by comparative genomic hybridization. The global transcriptome of whole blastocysts was analyzed with small cell number RNA sequencing, and they were compared to understand the gene expression behavior at early development and its imp…

0301 basic medicineAdultDown syndromeReproductive Techniques AssistedChromosomes Human Pair 21Embryonic DevelopmentBiologyTranscriptomeAndrologyEmbryo Culture Techniques03 medical and health sciences0302 clinical medicineMonosomyPregnancymedicineHumansBlastocystProspective StudiesGenetic Association Studies030219 obstetrics & reproductive medicineObstetrics and GynecologyEmbryomedicine.diseaseAneuploidy030104 developmental biologymedicine.anatomical_structureReproductive Medicineembryonic structuresFemalePloidyTrisomyChromosome 21TranscriptomeComparative genomic hybridizationFertility and sterility
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Differential metabolic profiling of non-pure trisomy 21 human preimplantation embryos.

2012

Objective To investigate the metabolomic signature of trisomy 21 preimplantation human embryos by a noninvasive approach using mass spectrometry– (MS-) and nuclear magnetic resonance spectroscopy– (NMR-) based metabolic profiling platforms. Design A total of 171 spent media samples were collected from day 3 embryos and comparatively analyzed by MS analysis (chromosomally normal embryos, n=15; trisomy 21 embryos, n=15) and a matched control media group (without embryo, n=14) and by NMR spectroscopy (normal embryos, n=39; trisomy 21 embryos, n=35; monosomy 21 embryos, n=24) and a matched control media group (without embryo, n=29). Setting IVF clinic/preimplantation genetic diagnosis (PGD) uni…

MaleDown syndromeSex Determination Analysisanimal structuresMagnetic Resonance SpectroscopyAneuploidyFertilization in VitroBiologyPreimplantation genetic diagnosisAndrosteroneGas Chromatography-Mass SpectrometryAndrologyEmbryo Culture Techniqueschemistry.chemical_compoundPredictive Value of TestsPregnancymedicineHumansMetabolomicsBlastocystCaproatesIn Situ Hybridization FluorescencePreimplantation DiagnosisGeneticsAndrosteroneObstetrics and GynecologyEmbryomedicine.diseaseAneuploidyCulture Mediamedicine.anatomical_structureBlastocystReproductive MedicinechemistryCase-Control Studiesembryonic structuresFeasibility StudiesFemaleDown SyndromeTrisomyChromosome 21BiomarkersChromatography LiquidFertility and sterility
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Obesity reduces uterine receptivity: clinical experience from 9,587 first cycles of ovum donation with normal weight donors

2013

Objective To analyze the reproductive outcome of recipients of donated ova according to their body mass index (BMI). Design Twelve-year retrospective cohort analysis. Setting Fertility clinics. Patient(s) 9,587 first cycles of ovum donation with ova from normoweight donors. Intervention(s) Recipients divided according to their BMI to analyze IVF laboratory and outcome parameters: lean with BMI 2 (n = 1,458; 15.2%); normoweight with BMI 20–24.9 kg/m 2 (n = 5,706; 59.5%), overweight with BMI 25–29.9 kg/m 2 (n = 1,770; 18.5%), and obese with BMI ≥30 kg/m 2 (n = 653; 6.8%). Main Outcome Measure(s) Implantation, biochemical and clinical pregnancy, miscarriage, and live-birth rates. Result(s) In …

Adultmedicine.medical_specialtyPregnancy RateFertilization in VitroOverweightBody Mass IndexMiscarriagePregnancyRisk FactorsOdds RatioHumansMedicineEmbryo ImplantationObesityTwin PregnancyRetrospective StudiesGynecologyAnalysis of VariancePregnancyChi-Square DistributionOocyte Donationbusiness.industryObstetricsBody WeightUterusnutritional and metabolic diseasesObstetrics and Gynecologymedicine.diseaseObesityTissue DonorsAbortion SpontaneousPregnancy rateLogistic ModelsTreatment OutcomeReproductive MedicineSpainPregnancy TwinFemalemedicine.symptomLive birthbusinessInfertility FemaleLive BirthBody mass indexFertility and Sterility
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